[Histonet] Pin4cocktail negative controls

Mon Feb 21 09:26:28 CST 2011

Hi Milton,

We use Biocare's Universal Negative Control Serum in place of the antibodies
in the negative control staining protocol.  We use normal prostate tissue to
show lack of p504s staining (to make sure it isn't overstaining normal
glands).  The prostate cancer in the tissue control shows lack of p63+ and
CK34BE12+ basal cells.

I hope this helps.

Mark
On Sun, Feb 20, 2011 at 12:00 PM, Gomez, Milton <Milton.Gomez <@t> nyumc.org>wrote:

> Hello Histonetters,
>
> What are you folks using as a negative control when running Pin4 cocktail
> antibody.
>
> Thanks in advance,
> MG
> </PRE>
> <html>
> <body>
> ------------------------------------------------------------<br />
> This email message, including any attachments, is for the sole use of the
> intended recipient(s) and may contain information that is proprietary,
> confidential, and exempt from disclosure under applicable law. Any
> unauthorized review, use, disclosure, or distribution is prohibited. If you
> have received this email in error please notify the sender by return email
> and delete the original message. Please note, the recipient should check
> this email and any attachments for the presence of viruses. The organization
> accepts no liability for any damage caused by any virus transmitted by this
> email.<br />
> =================================
> </body>
> </html>
> <PRE>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>