[Histonet] Pin4cocktail negative controls

Mark Tarango marktarango <@t> gmail.com
Mon Feb 21 09:26:28 CST 2011

Hi Milton,

We use Biocare's Universal Negative Control Serum in place of the antibodies
in the negative control staining protocol.  We use normal prostate tissue to
show lack of p504s staining (to make sure it isn't overstaining normal
glands).  The prostate cancer in the tissue control shows lack of p63+ and
CK34BE12+ basal cells.

I hope this helps.

On Sun, Feb 20, 2011 at 12:00 PM, Gomez, Milton <Milton.Gomez <@t> nyumc.org>wrote:

> Hello Histonetters,
> What are you folks using as a negative control when running Pin4 cocktail
> antibody.
> Thanks in advance,
> MG
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