[Histonet] RE: Pin4cocktail negative controls

Mon Feb 21 08:16:25 CST 2011

I assume you mean what reagent to use as the negative control?  The ideal control is a mixture of negative mouse serum and negative rabbit specifically titered to match the antibody concentration in the cocktail, which is probably proprietary. The next best solution is to use a universal negative control serum. The universal component typically denotes use with rabbit and mouse antibodies. 

Will Chappell, HTL(ASCP)QIHC
Histologist at large

Sent from my iPhone

On Feb 21, 2011, at 8:07 AM, Carol Bryant <cbrya <@t> lexclin.com> wrote:

> I would like that information also. 
> Thank you,
> Carol 
> 
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Gomez, Milton
> Sent: Sunday, February 20, 2011 3:00 PM
> To: 'histonet <@t> lists.utsouthwestern.edu'
> Subject: [Histonet] Pin4cocktail negative controls
> 
> Hello Histonetters,
> 
> What are you folks using as a negative control when running Pin4 cocktail antibody.
> 
> Thanks in advance,
> MG
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