SPAM-LOW: [Histonet] neutrophil antibody on rat FFP section

[Jan Shivers]

The website says it works on humans.  I have not tried this particular Ab 
myself, though I have tried another xNeutrophil Elastase.
Jan Shivers

----- Original Message ----- 
From: "Patsy Ruegg" <pruegg <@t> ihctech.net>
To: "'Jan Shivers'" <shive003 <@t> umn.edu>; "'Amy Lee'" <amylee779 <@t> yahoo.com>; 
"'histonet'" <histonet <@t> lists.utsouthwestern.edu>
Sent: Thursday, February 17, 2011 12:20 PM
Subject: RE: SPAM-LOW: [Histonet] neutrophil antibody on rat FFP section


> Does it also work on human?
>
> Patsy Ruegg, HT(ASCP)QIHC
> IHCtech, LLC
> Fitzsimmons BioScience Park
> 12635 Montview Blvd. Suite 215
> Aurora, CO 80010
> P-720-859-4060
> F-720-859-4110
> wk email pruegg <@t> ihctech.net
> web site www.ihctech.net
>
>
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> -----Original Message-----
> From: Jan Shivers [mailto:shive003 <@t> umn.edu]
> Sent: Thursday, February 17, 2011 9:39 AM
> To: Patsy Ruegg; 'Amy Lee'; 'histonet'
> Subject: Re: SPAM-LOW: [Histonet] neutrophil antibody on rat FFP section
>
> Abcam has a rabbit anti-neutrophil elastase that works on rat FFPE 
> tissues,
> according to their website.
>
> Jan Shivers
>
> ----- Original Message ----- 
> From: "Patsy Ruegg" <pruegg <@t> ihctech.net>
> To: "'Amy Lee'" <amylee779 <@t> yahoo.com>; "'histonet'"
> <histonet <@t> lists.utsouthwestern.edu>
> Sent: Thursday, February 17, 2011 8:49 AM
> Subject: RE: SPAM-LOW: [Histonet] neutrophil antibody on rat FFP section
>
>
> I do not know of a neutrophil specific ab for human ffpe tissue.  You 
> could
> do just DAB without blocking with h202 for myleoperoxidase but it will not
> distinguish between eosinophils and neutrophils myeloperoxidase will stain
> all myeloid cells with granules in the cytoplasm (granulocytes).
>
> Patsy
>
> Patsy Ruegg, HT(ASCP)QIHC
> IHCtech
> 12635 Montview Blvd. Ste.215
> Aurora, CO 80045
> 720-859-4060
> fax 720-859-4110
> www.ihctech.net
> www.ihcrg.org
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Amy Lee
> Sent: Wednesday, February 16, 2011 1:07 PM
> To: histonet
> Subject: SPAM-LOW: [Histonet] neutrophil antibody on rat FFP section
>
> Hello,
> I was asked to perform neutrophil IHC on rat FFP sections. I searched 
> around
> and found most are for mouse tissue. There is one from Serotec mouse anti
> rat but it does not work on paraffin section.
>
> Could anybody recommend a good antibody?
>
> Thanks in advance,
>
> Amy
>
>
>
>
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