SPAM-LOW: [Histonet] neutrophil antibody on rat FFP section

Patsy Ruegg pruegg <@t>
Thu Feb 17 12:20:57 CST 2011

Does it also work on human?

Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80010
wk email pruegg <@t>
web site

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-----Original Message-----
From: Jan Shivers [mailto:shive003 <@t>] 
Sent: Thursday, February 17, 2011 9:39 AM
To: Patsy Ruegg; 'Amy Lee'; 'histonet'
Subject: Re: SPAM-LOW: [Histonet] neutrophil antibody on rat FFP section

Abcam has a rabbit anti-neutrophil elastase that works on rat FFPE tissues, 
according to their website.

Jan Shivers

----- Original Message ----- 
From: "Patsy Ruegg" <pruegg <@t>>
To: "'Amy Lee'" <amylee779 <@t>>; "'histonet'" 
<histonet <@t>>
Sent: Thursday, February 17, 2011 8:49 AM
Subject: RE: SPAM-LOW: [Histonet] neutrophil antibody on rat FFP section

I do not know of a neutrophil specific ab for human ffpe tissue.  You could
do just DAB without blocking with h202 for myleoperoxidase but it will not
distinguish between eosinophils and neutrophils myeloperoxidase will stain
all myeloid cells with granules in the cytoplasm (granulocytes).


Patsy Ruegg, HT(ASCP)QIHC
12635 Montview Blvd. Ste.215
Aurora, CO 80045
fax 720-859-4110

-----Original Message-----
From: histonet-bounces <@t>
[mailto:histonet-bounces <@t>] On Behalf Of Amy Lee
Sent: Wednesday, February 16, 2011 1:07 PM
To: histonet
Subject: SPAM-LOW: [Histonet] neutrophil antibody on rat FFP section

I was asked to perform neutrophil IHC on rat FFP sections. I searched around
and found most are for mouse tissue. There is one from Serotec mouse anti
rat but it does not work on paraffin section.

Could anybody recommend a good antibody?

Thanks in advance,


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