SPAM-LOW: [Histonet] neutrophil antibody on rat FFP section

[Patsy Ruegg]

Does it also work on human?

Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
wk email pruegg <@t> ihctech.net
web site www.ihctech.net
 

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-----Original Message-----
From: Jan Shivers [mailto:shive003 <@t> umn.edu] 
Sent: Thursday, February 17, 2011 9:39 AM
To: Patsy Ruegg; 'Amy Lee'; 'histonet'
Subject: Re: SPAM-LOW: [Histonet] neutrophil antibody on rat FFP section

Abcam has a rabbit anti-neutrophil elastase that works on rat FFPE tissues, 
according to their website.

Jan Shivers

----- Original Message ----- 
From: "Patsy Ruegg" <pruegg <@t> ihctech.net>
To: "'Amy Lee'" <amylee779 <@t> yahoo.com>; "'histonet'" 
<histonet <@t> lists.utsouthwestern.edu>
Sent: Thursday, February 17, 2011 8:49 AM
Subject: RE: SPAM-LOW: [Histonet] neutrophil antibody on rat FFP section


I do not know of a neutrophil specific ab for human ffpe tissue.  You could
do just DAB without blocking with h202 for myleoperoxidase but it will not
distinguish between eosinophils and neutrophils myeloperoxidase will stain
all myeloid cells with granules in the cytoplasm (granulocytes).

Patsy

Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net
www.ihcrg.org

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Amy Lee
Sent: Wednesday, February 16, 2011 1:07 PM
To: histonet
Subject: SPAM-LOW: [Histonet] neutrophil antibody on rat FFP section

Hello,
I was asked to perform neutrophil IHC on rat FFP sections. I searched around
and found most are for mouse tissue. There is one from Serotec mouse anti
rat but it does not work on paraffin section.

Could anybody recommend a good antibody?

Thanks in advance,

Amy




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