[Histonet] RE: Histonet Digest, Vol 87, Issue 28
Ma, Minhui
mminhui <@t> emory.edu
Thu Feb 17 08:33:25 CST 2011
Dear everyone,
I'm wondering where I can order the Picro-Mallory Trichrome Stain kit? I couldn't find it online excepted some Italian companies.
Thanks,
Minhui Ma MD,HTL
Emory University
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: Wednesday, February 16, 2011 12:30 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 87, Issue 28
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Today's Topics:
1. RE: Hema 3/Diff Quik (Wanda.Smith <@t> HCAhealthcare.com)
2. HT Position in Oklahoma City (Gaiser, Marcia)
3. RE: Re: saliva for glycogen hydrolysis (Ingles Claire )
4. Wage question for Wisconsin (Michael Hillmer)
5. RE: Wage question for Wisconsin (Ingles Claire )
6. Re: Wage question for Wisconsin (Lee & Peggy Wenk)
7. Section position on slides (Tanya Ewing-Finchem)
8. Novolink Polymer detection kit (amitapandey <@t> torrentpharma.com)
9. Re: Section position on slides (Paula Sicurello)
10. Na K ATpase immunohistochemistry on rat FFPE tissue
(amitapandey <@t> torrentpharma.com)
11. RE: Wage question for Wisconsin (sgoebel <@t> mirnarx.com)
12. RE: Section position on slides (sgoebel <@t> mirnarx.com)
13. RE: Re: saliva for glycogen hydrolysis (Gill, Caula A.)
14. Re: Wage question for Wisconsin (Victoria Baker)
15. Correction, Correction on cutoff date for GSH meeting
(Shirley A. Powell)
16. Pathco double-blade handle (Breeden, Sara)
17. RE: Pathco double-blade handle (jstaruk)
18. Exam Prep (caymanfleck <@t> gmail.com)
19. RE: Exam Prep (Johnson, Nacaela)
20. RE: Exam Prep (sgoebel <@t> mirnarx.com)
21. Re: Section position on slides (Bill B.)
22. RE: Section position on slides
(Bartlett, Jeanine (CDC/OID/NCEZID))
23. RE: Section position on slides (Helen Fedor)
----------------------------------------------------------------------
Message: 1
Date: Tue, 15 Feb 2011 12:07:09 -0600
From: <Wanda.Smith <@t> HCAhealthcare.com>
Subject: [Histonet] RE: Hema 3/Diff Quik
To: <NKonop <@t> chw.org>, <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<9E2D36CE2D7CBA4A94D9B22E8328A3BA139EE804DD <@t> NADCWPMSGCMS03.hca.corpad.net>
Content-Type: text/plain; charset="us-ascii"
Good Afternoon,
We use:
88333 Touch prep initial site
88334 Touch prep addition site
WANDA G. SMITH, HTL(ASCP)HT
Pathology Supervisor
TRIDENT MEDICAL CENTER
9330 Medical Plaza Drive
Charleston, SC 29406
843-847-4586
843-847-4296 fax
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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Konop, Nicole
Sent: Tuesday, February 15, 2011 12:27 PM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] RE: Hema 3/Diff Quik
I should also include that we are using this diff quik as a rapid stain during frozen section in conjunction with a rapid H&E.
Nicole Anne Konop BS, HTL(ASCP)
Histology Team Lead
Children's Hospital of Wisconsin
(414)266-6580 Direct Line
(414)907-0366 Pager
(414)266-2524 Histology Department
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Konop, Nicole
Sent: Tuesday, February 15, 2011 11:01 AM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] Hema 3/Diff Quik
I'm looking for information on what CPT codes are being used for the diff quik/hema 3 stain? Any feedback is greatly appreciated. Thanks!
Nicole Anne Konop BS, HTL(ASCP)
Histology Team Lead
Children's Hospital of Wisconsin
(414)266-6580 Direct Line
(414)907-0366 Pager
(414)266-2524 Histology Department
_______________________________________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 2
Date: Tue, 15 Feb 2011 14:25:18 -0600
From: "Gaiser, Marcia" <Marcia_Gaiser <@t> ssmhc.com>
Subject: [Histonet] HT Position in Oklahoma City
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<728F817C02110E498D803A7C3B0C6248057D85BD5C <@t> S009-APEXM06.ds.ad.ssmhc.com>
Content-Type: text/plain; charset="Windows-1252"
St. Anthony Hospital currently has an excellent opportunity for an experienced Histologic Technician. This position requires Certification as an HT or HLT ? or ? experience acceptable to the Laboratory Director. Two years of previous histology experience required with IHC and/or grossing experience a plus. Outstanding benefits package, including generous paid time off. For consideration, please apply online at www.saintsok.com<http://www.saintsok.com/<https://mobile.ssmhc.com/owa/UrlBlockedError.aspx<https://mobile.ssmhc.com/owa/UrlBlockedError.aspx>>>, Ad # 10762, or contact Anna King for additional information at (405) 272-6105.
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------------------------------
Message: 3
Date: Tue, 15 Feb 2011 16:55:24 -0600
From: "Ingles Claire " <CIngles <@t> uwhealth.org>
Subject: RE: [Histonet] Re: saliva for glycogen hydrolysis
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<F2F030053F9B7345831BED293A6D57E103A1A6CE <@t> UWHC-MAIL01.uwhis.hosp.wisc.edu>
Content-Type: text/plain; charset="iso-8859-1"
Wouldn't GUM be a more palatable option? Who knows where some of those rubber bands have been!
Claire
________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Robert Richmond
Sent: Mon 2/14/2011 11:09 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Re: saliva for glycogen hydrolysis
Here's a truly hi-tech suggestion for doing PAS-diastase stains by the
spit method (which by the way is still how it's done by the small
pathology services I work on):
You can produce copious quantities of saliva by the simple expedient
of chewing on a rubber band for a couple of minutes. This used to be
the technique used to obtain saliva samples for determination of ABH
substance secretor status in the blood bank. (I belong to that elite
20% of donors who are non-secretors, and Lewis-a positive to prove
it.)
Bob Richmond
Samurai pathologist and occasional sialogogue
Knoxville TN
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 4
Date: Tue, 15 Feb 2011 17:02:58 -0600
From: "Michael Hillmer" <mhillmer <@t> dermwisconsin.com>
Subject: [Histonet] Wage question for Wisconsin
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<9060F1C2EB3D8A4AAF3B3433C9003230F975A8 <@t> hostexchbe1.hosting.tushausweb.com>
Content-Type: text/plain; charset="us-ascii"
We are dermatology clinic in Northeast Wisconsin and we are trying to do
gather accurate wage data. Can anybody offer wages for an HT, HtL and
lab assistants? Any help would be greatly appreciated.
Thank you-
Michael Hillmer PHR
HR Coordinator
Dermatology Associates of Wisconsin
Phone: (920)683-5278
Fax: (920)686-9674
Cell: (920)860-6360
The materials and information in this e-mail are confidential and may
contain Protected Health Information covered under the HIPAA Privacy
Rule. If you are not the intended recipient, be advised that any
unauthorized use, disclosure, copying, distribution, or action taken in
reliance on the contents of this information is strictly forbidden by
law. If you have received this e-mail in error, please notify me by
reply e-mail and then delete this message. Do not pass any of this
information to anyone else. Thank you for your cooperation.
------------------------------
Message: 5
Date: Tue, 15 Feb 2011 18:48:09 -0600
From: "Ingles Claire " <CIngles <@t> uwhealth.org>
Subject: RE: [Histonet] Wage question for Wisconsin
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<F2F030053F9B7345831BED293A6D57E103A1A6D2 <@t> UWHC-MAIL01.uwhis.hosp.wisc.edu>
Content-Type: text/plain; charset="iso-8859-1"
The answer to that question may have to wait a week or two. At least here at the UW. Our wonderful governor is trying to get rid of the state workers unions and collective bargaining. He is trying to push it through (it was just introduced this last Friday), and the vote is slated for later this week. The techs at the UW hospital have fortunately signed their contract that is good until the reopener in 1013 and we are not strictly state employees. However, the way things are looking, it is not out of the question that he may also try to void our contracts. Our pay MAY go up, but we will no longer have much, if anything, for benefits. Don't count on sending your kids to the UW colleges if this goes through.
Sorry, I'm fighting mad right now. I'm not usually big on the unions, but this is HUGE for millions of Wisconsinites. GGRRRRR!
Claire
Were there any other Wisconsin techs at the rally today? Even the Police and Firefighters unions were down there!
________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Michael Hillmer
Sent: Tue 2/15/2011 5:02 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Wage question for Wisconsin
We are dermatology clinic in Northeast Wisconsin and we are trying to do
gather accurate wage data. Can anybody offer wages for an HT, HtL and
lab assistants? Any help would be greatly appreciated.
Thank you-
Michael Hillmer PHR
HR Coordinator
Dermatology Associates of Wisconsin
Phone: (920)683-5278
------------------------------
Message: 6
Date: Tue, 15 Feb 2011 20:27:32 -0500
From: "Lee & Peggy Wenk" <lpwenk <@t> sbcglobal.net>
Subject: Re: [Histonet] Wage question for Wisconsin
To: "Michael Hillmer" <mhillmer <@t> dermwisconsin.com>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <75338FFC879A4718B6404F650270FE3C <@t> HP2010>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
reply-type=original
The following link is to the ASCP "Laboratory Medicine" article from March
2009. They do the survey every other year, so there should be another survey
coming out any month now.
http://www.ascp.org/pdf/Membership-Communications/Wage-and-Vacancy-Survey.aspx
Advance for Medical Laboratory Professionals just published their survey on
Jan. 31, 2011.
http://laboratorian.advanceweb.com/SharedResources/Downloads/2011/013111/MT013111_SalarySurvey.pdf
Realize that even with a state, there can be several dollars per hour
difference. Let alone the differences between states in a region. I suggest
taking the information in the surveys, look at the relationship between
HT/HTL, MLT/MT, and CT, and then apply the same ratio within your own
institute.
Hope that helps.
Peggy A. Wenk, HTL(ASCP)SLS
Beaumont Hospital
Royal Oak, MI 48073
--------------------------------------------------
From: "Michael Hillmer" <mhillmer <@t> dermwisconsin.com>
Sent: Tuesday, February 15, 2011 6:02 PM
To: <histonet <@t> lists.utsouthwestern.edu>
Subject: [Histonet] Wage question for Wisconsin
> We are dermatology clinic in Northeast Wisconsin and we are trying to do
> gather accurate wage data. Can anybody offer wages for an HT, HtL and
> lab assistants? Any help would be greatly appreciated.
>
>
>
>
>
>
>
>
>
> Thank you-
>
>
>
> Michael Hillmer PHR
>
> HR Coordinator
>
> Dermatology Associates of Wisconsin
>
> Phone: (920)683-5278
>
> Fax: (920)686-9674
>
> Cell: (920)860-6360
>
>
>
> The materials and information in this e-mail are confidential and may
> contain Protected Health Information covered under the HIPAA Privacy
> Rule. If you are not the intended recipient, be advised that any
> unauthorized use, disclosure, copying, distribution, or action taken in
> reliance on the contents of this information is strictly forbidden by
> law. If you have received this e-mail in error, please notify me by
> reply e-mail and then delete this message. Do not pass any of this
> information to anyone else. Thank you for your cooperation.
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 7
Date: Tue, 15 Feb 2011 22:09:24 -0700
From: Tanya Ewing-Finchem <tjey <@t> hotmail.com>
Subject: [Histonet] Section position on slides
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <BLU157-w10A8B2F000172BF782CF20CDD20 <@t> phx.gbl>
Content-Type: text/plain; charset="Windows-1252"
I am trying to put together a training document around microtomy and sectioning and am finding it hard to find information around the placement of the actual sections on the slides. These are the objectives I am looking to answer. Is this information found in any publications?
1) Tissue / Section Placement: Are there published guidelines / documentation on precisely where you should place tissue sections on a 25mm x 75mm glass slide? Perhaps more importantly, where you should NOT place tissue (ie. ?x? mm from the edge of the glass slide)?
2) Diagnosable Slide Staining Area: With automation becoming more widely used in IHC, are there published guidelines / documentation on the usable or diagnosable staining area on a 25mm x 75mm glass slide? For instance, would you define that as the area under a traditional coverslip? Would this be defined as the entire slide below the label? Or is this some distance from all the edges of the slide? With some automated systems, it is near impossible to get edge to edge staining. Is this acceptable?
Thanks for any ideas.
------------------------------
Message: 8
Date: Wed, 16 Feb 2011 11:39:14 +0530
From: amitapandey <@t> torrentpharma.com
Subject: [Histonet] Novolink Polymer detection kit
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<OF0B53C2D6.1196A49A-ON65257839.002127B5-65257839.0021CD79 <@t> torrentpharma.com>
Content-Type: text/plain; charset="US-ASCII"
Hello Histonetters,
This is first time, I am planning to use Novolink polymer detection kit
for my mouse monoclonal antibody (Millipore Anti Na K ATPase and anti
vimentin) and rabbit polyclonal antibody (Sc-1506R).
If any body has experience on this polymer kit , please share your
valuable experience which will help me to set up the method in my lab.
Thanks in advance
Amita
------------------------------
Message: 9
Date: Wed, 16 Feb 2011 07:02:58 -0500
From: Paula Sicurello <patpxs <@t> gmail.com>
Subject: Re: [Histonet] Section position on slides
To: Tanya Ewing-Finchem <tjey <@t> hotmail.com>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<AANLkTiksDVmFcb1J0Cgwn5iuUcGF9Cw4MWTVZ0vOfL9Z <@t> mail.gmail.com>
Content-Type: text/plain; charset=windows-1252
Hi Tanya,
It's always best to train people to place the section in the middle of
the slide. It depends if one has to put multiple sections on a slide.
Learning how to do proper placement will help them when they have to
cut controls for IHC that are often times placed on the top of the
slide.
Sections placed too close to the very bottom or top sometimes don't
get stained or coverslipped. Too close to the edges and you can
suffer wrap around or overlap, causing the section to be difficult for
the pathologist to read. Boy howdy! They will let you know if they
don't like the placement.
Therefore, train for centering the section as best as possible.
I hope this helps,
Paula :-)
On Wed, Feb 16, 2011 at 12:09 AM, Tanya Ewing-Finchem <tjey <@t> hotmail.com> wrote:
>
> I am trying to put together a training document around microtomy and sectioning and am finding it hard to find information around the placement of the actual sections on the slides. ?These are the objectives I am looking to answer. ?Is this information found in any publications?
>
> 1) ?Tissue / Section Placement: ?Are there published guidelines / documentation on precisely where you should place tissue sections on a 25mm x 75mm glass slide? ? Perhaps more importantly, where you should NOT place tissue (ie. ?x? mm from the edge of the glass slide)?
>
> 2) ?Diagnosable Slide Staining Area: ?With automation becoming more widely used in IHC, are there published guidelines / documentation on the usable or diagnosable staining area on a 25mm x 75mm glass slide? ?For instance, would you define that as the area under a traditional coverslip? ?Would this be defined as the entire slide below the label? ?Or is this some distance from all the edges of the slide? ?With some automated systems, it is near impossible to get edge to edge staining. ?Is this acceptable?
>
> Thanks for any ideas. ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
--
Paula Sicurello, HTL (ASCP)
Supervisor, Electron Microscope Laboratory
Duke University Health System
Rm.#251M, Duke South, Green Zone
Durham, North Carolina 27710
P: ?919.684.2091
------------------------------
Message: 10
Date: Wed, 16 Feb 2011 17:42:37 +0530
From: amitapandey <@t> torrentpharma.com
Subject: [Histonet] Na K ATpase immunohistochemistry on rat FFPE
tissue
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<OFF2BD856F.83823B44-ON65257839.0042A96B-65257839.00431316 <@t> torrentpharma.com>
Content-Type: text/plain; charset="US-ASCII"
Does any body have experienced on Anti Na K ATpase immunohistochemistry on
FFPE rat kidney tissue?
I am finding difficult to have any supportive lit regarding this, most of
them support to frozen sectioning.
Thanks a lot
Amita
------------------------------
Message: 11
Date: Wed, 16 Feb 2011 08:56:20 -0600
From: <sgoebel <@t> mirnarx.com>
Subject: RE: [Histonet] Wage question for Wisconsin
To: <CIngles <@t> uwhealth.org>, <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<D957F2A7D21959488C492A2680F9920A1C58F2 <@t> svrexch.asuragen.us>
Content-Type: text/plain; charset="US-ASCII"
At least you have a union!!
Sarah Goebel, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas 78744
(512)901-0900 ext. 6912
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Ingles
Claire
Sent: Tuesday, February 15, 2011 6:48 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Wage question for Wisconsin
The answer to that question may have to wait a week or two. At least
here at the UW. Our wonderful governor is trying to get rid of the state
workers unions and collective bargaining. He is trying to push it
through (it was just introduced this last Friday), and the vote is
slated for later this week. The techs at the UW hospital have
fortunately signed their contract that is good until the reopener in
1013 and we are not strictly state employees. However, the way things
are looking, it is not out of the question that he may also try to void
our contracts. Our pay MAY go up, but we will no longer have much, if
anything, for benefits. Don't count on sending your kids to the UW
colleges if this goes through.
Sorry, I'm fighting mad right now. I'm not usually big on the unions,
but this is HUGE for millions of Wisconsinites. GGRRRRR!
Claire
Were there any other Wisconsin techs at the rally today? Even the
Police and Firefighters unions were down there!
________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Michael
Hillmer
Sent: Tue 2/15/2011 5:02 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Wage question for Wisconsin
We are dermatology clinic in Northeast Wisconsin and we are trying to do
gather accurate wage data. Can anybody offer wages for an HT, HtL and
lab assistants? Any help would be greatly appreciated.
Thank you-
Michael Hillmer PHR
HR Coordinator
Dermatology Associates of Wisconsin
Phone: (920)683-5278
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 12
Date: Wed, 16 Feb 2011 08:59:44 -0600
From: <sgoebel <@t> mirnarx.com>
Subject: RE: [Histonet] Section position on slides
To: <tjey <@t> hotmail.com>, <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<D957F2A7D21959488C492A2680F9920A1C58F5 <@t> svrexch.asuragen.us>
Content-Type: text/plain; charset="US-ASCII"
I learned from an old school HT and studied using the Carson book. In
this book there are several pages on where to put sections. If you
don't have the Frieda Carson book (or "the bible" as I refer to it), get
it. A new edition just came out about a year ago. If you're an ASCP
member I think the price is reduced...
For the most part, just put everything as close to the middle of the
clear glass part of the slide as possible. This also does well for IHC
automation. I think most automated machines you can adjust the drop
range too if you want to put the sections on the bottom or top?
Good Luck!
Sarah Goebel, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas 78744
(512)901-0900 ext. 6912
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Tanya
Ewing-Finchem
Sent: Tuesday, February 15, 2011 11:09 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Section position on slides
I am trying to put together a training document around microtomy and
sectioning and am finding it hard to find information around the
placement of the actual sections on the slides. These are the
objectives I am looking to answer. Is this information found in any
publications?
1) Tissue / Section Placement: Are there published guidelines /
documentation on precisely where you should place tissue sections on a
25mm x 75mm glass slide? Perhaps more importantly, where you should
NOT place tissue (ie. "x" mm from the edge of the glass slide)?
2) Diagnosable Slide Staining Area: With automation becoming more
widely used in IHC, are there published guidelines / documentation on
the usable or diagnosable staining area on a 25mm x 75mm glass slide?
For instance, would you define that as the area under a traditional
coverslip? Would this be defined as the entire slide below the label?
Or is this some distance from all the edges of the slide? With some
automated systems, it is near impossible to get edge to edge staining.
Is this acceptable?
Thanks for any ideas.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 13
Date: Wed, 16 Feb 2011 10:15:29 -0500
From: "Gill, Caula A." <cgill <@t> marylandgeneral.org>
Subject: RE: [Histonet] Re: saliva for glycogen hydrolysis
To: "Ingles Claire " <CIngles <@t> uwhealth.org>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<087A9911BBAFDE4B8151CB148586E2C23A9F38 <@t> MDGEN-EXCH1.marylandgeneral.org>
Content-Type: text/plain; charset="us-ascii"
I agree, gum sounds better not to mention the taste
CG
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Ingles
Claire
Sent: Tuesday, February 15, 2011 5:55 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Re: saliva for glycogen hydrolysis
Wouldn't GUM be a more palatable option? Who knows where some of those
rubber bands have been!
Claire
________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Robert
Richmond
Sent: Mon 2/14/2011 11:09 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Re: saliva for glycogen hydrolysis
Here's a truly hi-tech suggestion for doing PAS-diastase stains by the
spit method (which by the way is still how it's done by the small
pathology services I work on):
You can produce copious quantities of saliva by the simple expedient of
chewing on a rubber band for a couple of minutes. This used to be the
technique used to obtain saliva samples for determination of ABH
substance secretor status in the blood bank. (I belong to that elite 20%
of donors who are non-secretors, and Lewis-a positive to prove
it.)
Bob Richmond
Samurai pathologist and occasional sialogogue Knoxville TN
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 14
Date: Wed, 16 Feb 2011 10:25:27 -0500
From: Victoria Baker <bakevictoria <@t> gmail.com>
Subject: Re: [Histonet] Wage question for Wisconsin
To: Michael Hillmer <mhillmer <@t> dermwisconsin.com>
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<AANLkTinj-abey-jrMWh2MjtKe+YWj4oqJwtwNW4+Cx6K <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Michael,
ASCP has a wage/salary survey that they distribute based upon region and
certification. Some have said that this is biased as not everyone does
participate. I tend to agree with the survey, but also recognize that
dermatopathology is a 'specialty' type lab. I worked in Dermpath at NYU
back in the 80's to early 90's and our salaries were not identical to the
clinical labs as we were a part of the medical school and not the hospital.
Yes the hospital was 1199 at that time, I cannot say if they are still.
My recommendation is to go with what you can find from the survey, but also
network with other dermpath or other specialty labs in your area that you
may know or who you can connect with through others - either on the
listserver or off - to possibly get a better sounding of salaries. HR at
your hospital might be also able to help if you have someone there that you
can work with and trust to obtain some of the data you're looking for and
understands what your specific needs are within the lab. I would include
your pathologists in this as much as possible as they will be needed to
support your request(s) as they are essentially your clients.
Vikki
On Tue, Feb 15, 2011 at 6:02 PM, Michael Hillmer <mhillmer <@t> dermwisconsin.com
> wrote:
> We are dermatology clinic in Northeast Wisconsin and we are trying to do
> gather accurate wage data. Can anybody offer wages for an HT, HtL and
> lab assistants? Any help would be greatly appreciated.
>
>
>
>
>
>
>
>
>
> Thank you-
>
>
>
> Michael Hillmer PHR
>
> HR Coordinator
>
> Dermatology Associates of Wisconsin
>
> Phone: (920)683-5278
>
> Fax: (920)686-9674
>
> Cell: (920)860-6360
>
>
>
> The materials and information in this e-mail are confidential and may
> contain Protected Health Information covered under the HIPAA Privacy
> Rule. If you are not the intended recipient, be advised that any
> unauthorized use, disclosure, copying, distribution, or action taken in
> reliance on the contents of this information is strictly forbidden by
> law. If you have received this e-mail in error, please notify me by
> reply e-mail and then delete this message. Do not pass any of this
> information to anyone else. Thank you for your cooperation.
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
------------------------------
Message: 15
Date: Wed, 16 Feb 2011 10:27:55 -0500
From: "Shirley A. Powell" <POWELL_SA <@t> mercer.edu>
Subject: [Histonet] Correction, Correction on cutoff date for GSH
meeting
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<9BF995BC0E47744E9673A41486E24EE22DBB3A7DE3 <@t> MERCERMAIL.MercerU.local>
Content-Type: text/plain; charset="us-ascii"
Correction: There is a correction on the cutoff date for the hotel reservations. Cutoff date for discounted room rate is February 23rd, Not March 1st. There are several other events going on that weekend and the hotel is filling up fast. Please make your reservations and register soon so you will not miss out on the great room rate and a great meeting.
Shirley Powell
Hi Georgia, Alabama, ALL histotechs,
The Georgia Society for Histotechnology invites you to our meeting March 25-27, 2011 at Callaway Gardens in Pine Mountain, Georgia which is near Columbus, Ga. and very convenient to Alabama folks, so come across the line. The invitation extends to any other states as well. Callaway Gardens is a fantastic site for family vacations, golf lovers, nature lovers, so come to Georgia for a visit and take in a wealth of histology knowledge.
The deadline for making hotel reservations is March 1, 2011 so that gives you a month to make your plans to attend, don't delay. The Mountain Creek Inn, Callaway Gardens, Pine Mountain, Georgia is the location and you can call for hotel reservations at 1-800-225-5292. Room rates start at $99 which includes Continental Breakfast and Admission to the Park. For more information about things to do at Callaway click on the link here: http://www.callawaygardens.com/resort/things-to-do/georgia-fun.aspx
Our theme this year is "METAMORPHOSIS: Transforming Histotechs." The complete program can be downloaded from our website at this link: www.histosearch.com/gsh<http://www.histosearch.com/gsh<http://www.histosearch.com/gsh%3chttp:/www.histosearch.com/gsh>> then click on GSH symposium link at the bottom of the home page. There you will find the complete program with registration form. The vendor registration form is on the same page for any last minute vendors who want to exhibit at our meeting. If anyone has questions, please contact me for assistance.
Come TRANSFORM yourselves.
Shirley Powell
GSH Secretary
Shirley A. Powell, HT(ASCP)HTL, QIHC
Technical Director
Histology Curricular Support Laboratory
Mercer University School of Medicine
1550 College Street
Macon, GA 31207
478-301-2374 Lab
478-301-5489 Fax
------------------------------
Message: 16
Date: Wed, 16 Feb 2011 08:59:36 -0700
From: "Breeden, Sara" <sbreeden <@t> nmda.nmsu.edu>
Subject: [Histonet] Pathco double-blade handle
To: "Histonet" <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<4D14F0FC9316DD41972D5F03C070908B02E4766A <@t> nmdamailsvr.nmda.ad.nmsu.edu>
Content-Type: text/plain; charset="us-ascii"
Am looking for this item to replace one that was older than I am. I
need the handle (perhaps Pathco #D2877) for the blades (Pathco
#D2878-2C). The catch is that I want the SOLID BRASS handle - not the
aluminum. I've "googled" all over the place - can anyone help me find
these handles? Muchas gracias!
Sally Breeden, HT(ASCP)
New Mexico Department of Agriculture
Veterinary Diagnostic Services
1101 Camino de Salud NE
Albuquerque, NM 87102
505-383-9278 (Histology Lab)
------------------------------
Message: 17
Date: Wed, 16 Feb 2011 11:09:31 -0500
From: "jstaruk" <jstaruk <@t> masshistology.com>
Subject: RE: [Histonet] Pathco double-blade handle
To: "'Breeden, Sara'" <sbreeden <@t> nmda.nmsu.edu>, "'Histonet'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <005501cbcdf3$e623bd40$b26b37c0$@masshistology.com>
Content-Type: text/plain; charset="us-ascii"
I've found two brass handles on Ebay in the last few years. I don't know if
you're aware of this but the exact same blades that fit these handles can be
purchased at Home Depot in the carpet section. $10 for 50 of them.
Jim
_______________________
James E. Staruk HT(ASCP)
<http://www.masshistology.com> www.masshistology.com
www.nehorselabs.com
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Breeden,
Sara
Sent: Wednesday, February 16, 2011 11:00 AM
To: Histonet
Subject: [Histonet] Pathco double-blade handle
Am looking for this item to replace one that was older than I am. I
need the handle (perhaps Pathco #D2877) for the blades (Pathco
#D2878-2C). The catch is that I want the SOLID BRASS handle - not the
aluminum. I've "googled" all over the place - can anyone help me find
these handles? Muchas gracias!
Sally Breeden, HT(ASCP)
New Mexico Department of Agriculture
Veterinary Diagnostic Services
1101 Camino de Salud NE
Albuquerque, NM 87102
505-383-9278 (Histology Lab)
_______________________________________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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------------------------------
Message: 18
Date: Wed, 16 Feb 2011 11:12:00 -0500
From: "caymanfleck <@t> gmail.com" <caymanfleck <@t> gmail.com>
Subject: [Histonet] Exam Prep
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<AANLkTimyreMDoNo+U3ApAsbhJZPnhstLqxCDyarPo+61 <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Hello Histoland,
I'm preparing for the HTL exam after being trained on-the-job and working in
histo for a few years. I'm using the standard 'Theory and practice...' text
as my main resource. I'm wondering if anyone has used either the ASCP
practice tests (a set of 5 are available from ascp for about $30) or the NSH
Self-assessment Program (which has modules that correspond to the exam
sections). The NSH program is expensive at about $240 but I don't know if
it's worth it. Has anyone used either of these resources?
Thanks!
------------------------------
Message: 19
Date: Wed, 16 Feb 2011 10:23:52 -0600
From: "Johnson, Nacaela" <Nacaela.Johnson <@t> USONCOLOGY.COM>
Subject: RE: [Histonet] Exam Prep
To: <caymanfleck <@t> gmail.com>, <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<6DBD71C31D7E444482E5D3DFBC202D260245D539 <@t> txhous1eb012.uson.usoncology.int>
Content-Type: text/plain; charset="us-ascii"
I just used the Freida Carson Histotechnology A Self-Instructional Test.
Thanks,
Nacaela Johnson, HTL (ASCP)
Histotechnologist
KCCC Pathology
12000 110th St., Ste. 400
Overland Park, KS 66210
Office: 913-234-0576
Fax: 913-433-7639
Email: Nacaela.Johnson <@t> USOncology.com
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
caymanfleck <@t> gmail.com
Sent: Wednesday, February 16, 2011 10:12 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Exam Prep
Hello Histoland,
I'm preparing for the HTL exam after being trained on-the-job and
working in histo for a few years. I'm using the standard 'Theory and
practice...' text as my main resource. I'm wondering if anyone has used
either the ASCP practice tests (a set of 5 are available from ascp for
about $30) or the NSH Self-assessment Program (which has modules that
correspond to the exam sections). The NSH program is expensive at about
$240 but I don't know if it's worth it. Has anyone used either of these
resources?
Thanks!
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
</pre>The contents of this electronic mail message and any attachments are confidential, possibly privileged and intended for the addressee(s) only.<br>Only the addressee(s) may read, disseminate, retain or otherwise use this message. If received in error, please immediately inform the sender and then delete this message without disclosing its contents to anyone.</pre>
------------------------------
Message: 20
Date: Wed, 16 Feb 2011 10:35:21 -0600
From: <sgoebel <@t> mirnarx.com>
Subject: RE: [Histonet] Exam Prep
To: <Nacaela.Johnson <@t> USONCOLOGY.COM>, <caymanfleck <@t> gmail.com>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<D957F2A7D21959488C492A2680F9920A1C5906 <@t> svrexch.asuragen.us>
Content-Type: text/plain; charset="US-ASCII"
If you basically memorize this book, and then have a pretty good
understanding of IHC...then you should do fine.
Sarah Goebel, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas 78744
(512)901-0900 ext. 6912
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Johnson,
Nacaela
Sent: Wednesday, February 16, 2011 10:24 AM
To: caymanfleck <@t> gmail.com; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Exam Prep
I just used the Freida Carson Histotechnology A Self-Instructional Test.
Thanks,
Nacaela Johnson, HTL (ASCP)
Histotechnologist
KCCC Pathology
12000 110th St., Ste. 400
Overland Park, KS 66210
Office: 913-234-0576
Fax: 913-433-7639
Email: Nacaela.Johnson <@t> USOncology.com
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
caymanfleck <@t> gmail.com
Sent: Wednesday, February 16, 2011 10:12 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Exam Prep
Hello Histoland,
I'm preparing for the HTL exam after being trained on-the-job and
working in histo for a few years. I'm using the standard 'Theory and
practice...' text as my main resource. I'm wondering if anyone has used
either the ASCP practice tests (a set of 5 are available from ascp for
about $30) or the NSH Self-assessment Program (which has modules that
correspond to the exam sections). The NSH program is expensive at about
$240 but I don't know if it's worth it. Has anyone used either of these
resources?
Thanks!
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
</pre>The contents of this electronic mail message and any attachments
are confidential, possibly privileged and intended for the addressee(s)
only.<br>Only the addressee(s) may read, disseminate, retain or
otherwise use this message. If received in error, please immediately
inform the sender and then delete this message without disclosing its
contents to anyone.</pre>
_______________________________________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 21
Date: Wed, 16 Feb 2011 11:08:07 -0600
From: "Bill B." <bill501 <@t> mindspring.com>
Subject: Re: [Histonet] Section position on slides
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <p06240801c981b14a90ef@[4.244.63.90]>
Content-Type: text/plain; charset="iso-8859-1"
As a pathologist I have some preferences on how certain tissues are oriented on a slide and work with my histotechs so they can learn my prefs. I also like my slides in certain orientation in the slide tray. EG, for orientation of skin, I like the the dermis at the top when I am looking at the slide. Some other tissues can be so oriented, some cannot. We often put multiple levels on one slide and I would prefer them to be all oriented similarly and in a straight line.
The most annoying thing I see beyond coverslipping the wrong side, is the label covering a part of the specimen. I don't care how close to the edge a section is as long as it is all on the slide and covered by the cover-slip or mounting medium.
I have no idea is these preferences are weird. My point is the answer to you 1st question may depend on your particular pathologists' preferences.
BIll Blank
At 10:09 PM -0700 2/15/11, Tanya Ewing-Finchem wrote:
>1) Tissue / Section Placement: Are there published guidelines / documentation on precisely where you should place tissue sections on a 25mm x 75mm glass slide? Perhaps more importantly, where you should NOT place tissue (ie. ?x? mm from the edge of the glass slide)?
------------------------------
Message: 22
Date: Wed, 16 Feb 2011 17:20:57 +0000
From: "Bartlett, Jeanine (CDC/OID/NCEZID)" <jqb7 <@t> cdc.gov>
Subject: RE: [Histonet] Section position on slides
To: Tanya Ewing-Finchem <tjey <@t> hotmail.com>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<DF1CBA3D83D9A344A7D6A045188E448405A9A2 <@t> EMBX-CLFT1.cdc.gov>
Content-Type: text/plain; charset="us-ascii"
I always try to center the section equally from sides and top-bottom as possible. This means measure from the bottom of the frosted edge as the "top". The Artisan special stains system has a clip that attaches around the slide to allow reagents to pool onto the sections and incubate. If the section is too close to the sides then these areas do not stain adequately. Sometimes if the tissue section itself is very large this is unavoidable. With automated coverslippers you must also consider placement of tissue to allow for proper coverage.
If I am cutting multiple unstained slides for subsequent testing I try to orient the tissue the same on each slide to facilitate the reading of these slides by the pathologist's
Jeanine Bartlett
Infectious Diseases Pathology Branch
(404) 639-3590
jeanine.bartlett <@t> cdc.hhs.gov
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Tanya Ewing-Finchem
Sent: Wednesday, February 16, 2011 12:09 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Section position on slides
I am trying to put together a training document around microtomy and sectioning and am finding it hard to find information around the placement of the actual sections on the slides. These are the objectives I am looking to answer. Is this information found in any publications?
1) Tissue / Section Placement: Are there published guidelines / documentation on precisely where you should place tissue sections on a 25mm x 75mm glass slide? Perhaps more importantly, where you should NOT place tissue (ie. "x" mm from the edge of the glass slide)?
2) Diagnosable Slide Staining Area: With automation becoming more widely used in IHC, are there published guidelines / documentation on the usable or diagnosable staining area on a 25mm x 75mm glass slide? For instance, would you define that as the area under a traditional coverslip? Would this be defined as the entire slide below the label? Or is this some distance from all the edges of the slide? With some automated systems, it is near impossible to get edge to edge staining. Is this acceptable?
Thanks for any ideas. _______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 23
Date: Wed, 16 Feb 2011 12:27:32 -0500
From: Helen Fedor <hfedor <@t> jhmi.edu>
Subject: RE: [Histonet] Section position on slides
To: "Bartlett, Jeanine (CDC/OID/NCEZID)" <jqb7 <@t> cdc.gov>, Tanya
Ewing-Finchem <tjey <@t> hotmail.com>, "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<DC2D3533BC05784793C2FCE01FCFB9260539A47E2B <@t> JHEMTEXVS3.win.ad.jhu.edu>
Content-Type: text/plain; charset="iso-8859-1"
Since automation is becoming more and more a part of all Histology labs the demands of placement of the tissue on the slides varies for different instruments. Stainers, coverslippers and now with slide scanning as well. So I do not believe that there is a silver bullet answer.
Helen L. Fedor
Tissue Microarray Lab, Manager
Prostate Spore Lab, Manager
Johns Hopkins University
600 N. Wolfe St,?| Marburg Room 406
Baltimore, MD?| 21287-7065
410.614.1660
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/OID/NCEZID)
Sent: Wednesday, February 16, 2011 12:21 PM
To: Tanya Ewing-Finchem; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Section position on slides
I always try to center the section equally from sides and top-bottom as possible. This means measure from the bottom of the frosted edge as the "top". The Artisan special stains system has a clip that attaches around the slide to allow reagents to pool onto the sections and incubate. If the section is too close to the sides then these areas do not stain adequately. Sometimes if the tissue section itself is very large this is unavoidable. With automated coverslippers you must also consider placement of tissue to allow for proper coverage.
If I am cutting multiple unstained slides for subsequent testing I try to orient the tissue the same on each slide to facilitate the reading of these slides by the pathologist's
Jeanine Bartlett
Infectious Diseases Pathology Branch
(404) 639-3590
jeanine.bartlett <@t> cdc.hhs.gov
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Tanya Ewing-Finchem
Sent: Wednesday, February 16, 2011 12:09 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Section position on slides
I am trying to put together a training document around microtomy and sectioning and am finding it hard to find information around the placement of the actual sections on the slides. These are the objectives I am looking to answer. Is this information found in any publications?
1) Tissue / Section Placement: Are there published guidelines / documentation on precisely where you should place tissue sections on a 25mm x 75mm glass slide? Perhaps more importantly, where you should NOT place tissue (ie. "x" mm from the edge of the glass slide)?
2) Diagnosable Slide Staining Area: With automation becoming more widely used in IHC, are there published guidelines / documentation on the usable or diagnosable staining area on a 25mm x 75mm glass slide? For instance, would you define that as the area under a traditional coverslip? Would this be defined as the entire slide below the label? Or is this some distance from all the edges of the slide? With some automated systems, it is near impossible to get edge to edge staining. Is this acceptable?
Thanks for any ideas. _______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
End of Histonet Digest, Vol 87, Issue 28
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