[Histonet] Section position on slides

Tanya Ewing-Finchem tjey <@t> hotmail.com
Tue Feb 15 23:09:24 CST 2011

I am trying to put together a training document around microtomy and sectioning and am finding it hard to find information around the placement of the actual sections on the slides.  These are the objectives I am looking to answer.  Is this information found in any publications?
1)  Tissue / Section Placement:  Are there published guidelines / documentation on precisely where you should place tissue sections on a 25mm x 75mm glass slide?   Perhaps more importantly, where you should NOT place tissue (ie. “x” mm from the edge of the glass slide)?
2)  Diagnosable Slide Staining Area:  With automation becoming more widely used in IHC, are there published guidelines / documentation on the usable or diagnosable staining area on a 25mm x 75mm glass slide?  For instance, would you define that as the area under a traditional coverslip?  Would this be defined as the entire slide below the label?  Or is this some distance from all the edges of the slide?  With some automated systems, it is near impossible to get edge to edge staining.  Is this acceptable?  
Thanks for any ideas. 		 	   		  

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