[Histonet] Re: saliva for glycogen hydrolysis

Ingles Claire CIngles <@t> uwhealth.org
Tue Feb 15 16:55:24 CST 2011

Wouldn't GUM be a more palatable option? Who knows where some of those rubber bands have been!


From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Robert Richmond
Sent: Mon 2/14/2011 11:09 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Re: saliva for glycogen hydrolysis

Here's a truly hi-tech suggestion for doing PAS-diastase stains by the
spit method (which by the way is still how it's done by the small
pathology services I work on):

You can produce copious quantities of saliva by the simple expedient
of chewing on a rubber band for a couple of minutes. This used to be
the technique used to obtain saliva samples for determination of ABH
substance secretor status in the blood bank. (I belong to that elite
20% of donors who are non-secretors, and Lewis-a positive to prove

Bob Richmond
Samurai pathologist and occasional sialogogue
Knoxville TN

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