[Histonet] processing histogel

[Makhijani, Nalini S]

You could try asking the authors of the paper for their protocol.

Nalini

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Luciana
Bastos
Sent: Monday, February 14, 2011 5:06 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] processing histogel



Hello,
We are working with cell culture embedded into three dimensional (3D) of
collagen gel type I.  The samples were fixed in 10% formalin for 24 h.
Even after fixation, the gel used in our preparation was too soft to be
embedded directly in paraffin. To circumvent this problem, we wold like
to dehydrated and embedded the samples in Histogel (Perk Scientific
Inc., Devon, PA, USA).11 V.M. Freitas and R.G. Jaeger, The effect of
laminin and its peptide SIKVAV on a human salivary gland adenoid cystic
carcinoma cell line, Virchows Arch 441 (6) (2002), pp. 569-576. Full
Text via CrossRef | View Record in Scopus | Cited By in Scopus (12)11
However, we need some help to how dehydrated the collagen gel and the
histogel (solutions, concetration, time, ect.) toparaffin-embedded and
stained by haematoxylin-eosin (H&E).
Thank you,
Luciana


 
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