[Histonet] Decal Solution for Bone Marrows

[Gervaip <@t> aol.com]

I neglected to say that the iron stain does not work well on decaled  
specimens.  We perform iron stains on bone marrow clots and smears .
 
 
In a message dated 2/11/2011 2:11:23 P.M. Central Standard Time,  
trathborne <@t> somerset-healthcare.com writes:

Decalcifier  II from Surgipath.

-----Original Message-----
From:  histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On  Behalf Of Marcia
Fisher
Sent: Friday, February 11, 2011 2:07 PM
To:  histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Decal Solution for  Bone Marrows


I would be most interested in finding out what other  labs are using to
decal the bone marrow biopsy cores.  The current  decal solution we are
using is too strong and affecting the Fe stains and  some IHC stains.
The decal solution we use for regular bone is great.   Routinely we decal
the core for one hour.  We tried 45 minutes but the  cores are still too
crunchy.  And I prefer not to face off the block  and float on 5% HCl for
20 minutes.  Thanking you in  advance.



M. Fisher

El Centro Regional Medical  Center



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