[Histonet] Decal Solution for Bone Marrows

Gervaip <@t> aol.com Gervaip <@t> aol.com
Fri Feb 11 20:57:47 CST 2011


We use the IEC from Biocare.  The bone biopsies are fixed for  a  minimum 
of 2 hours and decaled in the IEC for two hours.  Sections great  and 
immunohistochemistry stains are beautiful.    
 
 
In a message dated 2/11/2011 2:11:23 P.M. Central Standard Time,  
trathborne <@t> somerset-healthcare.com writes:

Decalcifier  II from Surgipath.

-----Original Message-----
From:  histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On  Behalf Of Marcia
Fisher
Sent: Friday, February 11, 2011 2:07 PM
To:  histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Decal Solution for  Bone Marrows


I would be most interested in finding out what other  labs are using to
decal the bone marrow biopsy cores.  The current  decal solution we are
using is too strong and affecting the Fe stains and  some IHC stains.
The decal solution we use for regular bone is great.   Routinely we decal
the core for one hour.  We tried 45 minutes but the  cores are still too
crunchy.  And I prefer not to face off the block  and float on 5% HCl for
20 minutes.  Thanking you in  advance.



M. Fisher

El Centro Regional Medical  Center



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