[Histonet] failed thionin staining part 2
An.Eerdekens <@t> med.kuleuven.be
Wed Feb 9 04:52:35 CST 2011
Dear friends of Histonet,
I have send already a message last week considering problems with thionin staining. I got a lot of protocols, thank you for that. We used different kind of solutions, but the problem remains, independently of the solution: when our samples go through the ethanol (50%, 70%, 90%, 100%) the thionin is completely washed of.
Has anyone got the same experience? What to do about it?
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