[Histonet] TTF-1/background staining

[Mark Tarango]

Hi Paula,

You mentioned the staining in a liver specimen using the cell marque
antibody against TTF-1.  The clone named 8G7G3/1 that cell marque sells is
known to stain liver cells and liver cancer.  Your pathologist might be
interested in knowing that.  Here is a reference you can give him:
http://www.nordiqc.org/Run-23-B5/Assessment/Assessment-TTF-1.htm   It also
lists another clone that doesn't cross react with liver.

Mark

On Thu, Feb 3, 2011 at 12:38 PM, Paula Lucas <plucas <@t> biopath.org> wrote:

> How can I eliminate the background or as you say, non-specific staining?
>
>
>
> I'm no expert here, I admit, and so I'm asking for your help and
> suggestions.  I have searched the Histonet archives, and a lot of what I'm
> seeing deals with animal or rodent tissues, and a lot of it was confusing
> to
> me.
>
>
>
> To give you a little background info:
>
> We use the Lab Vision stainer and the TTF-1 antibody we use is from Cell
> Marque.  We were having issues with this marker from Lab Vision, so we
> switched to TTF-1 a while ago.  We use the UltraVision LP detection kit
> from
> Lab Vision. It's a polymer driven detection kit.
>
>
>
> The antibody is a ready to use, and we have the time set at 30 minutes.
>
>
>
> We were getting the background staining on a liver specimen last week, and
> we also had this problem today on a lung case.  My doctor is getting
> frustrated, and wants me to do something about this, and to repeat the
> TTF-1
> stain on the lung, so if someone can give me some suggestions to try, I'm
> ready to try them.
>
>
>
> Thanks in advance,
>
> Paula
>
> Lab Manager
>
> Bio-Path Medical Group
>
> Fountain Valley, CA
>
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