[Histonet] Her2 Fixation Requirement

[Chiriboga, Luis]

I believe the two most recent articles from ASCO/CAP on this subject are

Hammond, M.E., D.F. Hayes, M. Dowsett, D.C. Allred, K.L. Hagerty, S. Badve, P.L. Fitzgibbons, G. Francis, et al. 2010. American Society of Clinical Oncology/College Of American Pathologists guideline recommendations for immunohistochemical testing of estrogen and progesterone receptors in breast cancer. J Clin Oncol 28:2784-2795.

Wolff, A.C., M.E. Hammond, J.N. Schwartz, K.L. Hagerty, D.C. Allred, R.J. Cote, M. Dowsett, P.L. Fitzgibbons, et al. 2007. American Society of Clinical Oncology/College of American Pathologists guideline recommendations for human epidermal growth factor receptor 2 testing in breast cancer. J Clin Oncol 25:118-145.

If you do not have access to the literature you can request reprints from the authors or it may be available on the ASCO website?

Luis









-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Liz Chlipala
Sent: Wednesday, February 02, 2011 2:28 PM
To: Lori.Disher <@t> HCAhealthcare.com; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Her2 Fixation Requirement

Lori

 

I would think both if you could since the Her2 paper does address those
issues as potentials for problems in testing variation (time to
fixation).  I believe the CAP/ASCO paper states not less then 6 and no
more than 48 hours for fixation (2007 paper).  I believe you also should
document type of fixative if possible; vendor, lot number and expiration
date if you can.  I have pdfs of these documents if you need them.

 

Liz

 

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC

Manager

Premier Laboratory, LLC

PO Box 18592

Boulder, Colorado 80308

office (303) 682-3949 

fax (303) 682-9060

www.premierlab.com

 

 

Ship to Address:

1567 Skyway Drive, Unit E

Longmont, Colorado 80504

 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Lori.Disher <@t> HCAhealthcare.com
Sent: Wednesday, February 02, 2011 12:16 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Her2 Fixation Requirement

 

  What is the appropriate time it should be in formalin?  Do you start
your time when it is initially put into formalin, or the start from the
time it was dissected?

 

Lori A Disher

Fawcett Memorial Hospital

Port Charlotte,  FL   33952

lori.disher <@t> hcahealthcare.com

 

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