[Histonet] Her2 Fixation Requirement

Liz Chlipala liz <@t> premierlab.com
Wed Feb 2 13:27:57 CST 2011



I would think both if you could since the Her2 paper does address those
issues as potentials for problems in testing variation (time to
fixation).  I believe the CAP/ASCO paper states not less then 6 and no
more than 48 hours for fixation (2007 paper).  I believe you also should
document type of fixative if possible; vendor, lot number and expiration
date if you can.  I have pdfs of these documents if you need them.




Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC


Premier Laboratory, LLC

PO Box 18592

Boulder, Colorado 80308

office (303) 682-3949 

fax (303) 682-9060




Ship to Address:

1567 Skyway Drive, Unit E

Longmont, Colorado 80504


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Lori.Disher <@t> HCAhealthcare.com
Sent: Wednesday, February 02, 2011 12:16 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Her2 Fixation Requirement


  What is the appropriate time it should be in formalin?  Do you start
your time when it is initially put into formalin, or the start from the
time it was dissected?


Lori A Disher

Fawcett Memorial Hospital

Port Charlotte,  FL   33952

lori.disher <@t> hcahealthcare.com



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