[Histonet] Question about Bile Stain Control tissue

[Kim Donadio]

Have you tried gall bladder?  And some  times livers that are diseased have it. They always look green. 
Just a thought.  
Sent from my iPhone

On Dec 20, 2011, at 3:45 PM, "Thurby, Christina" <christina.thurby <@t> bms.com> wrote:

> Hello All,
> I have a few questions about Bile Stain control tissue:  First - does anyone have or know of any good sources where I can purchase a couple of positive control blocks demonstrating bile in the tissue?  I have some purchased control slides - but they appear to be from 'bile sludge' prepared to block.  This is a nice emerald green when stained with the Hall's method, but our pathologists would like to see positive bile demonstrated in the tissue elements.  Carson's newest book has a very nice image.
> 
> Next question:  Carson's procedure for the Hall's bile stain describes the positive elements as a emerald green to drab olive in color.  We have a case that appears to be positive but the Hall's stain is kind of a camouflage brownish/drab olive green.  Is there a chance we're doing something wrong with this procedure?  Our purchased 'bile sludge' control slides are a nice emerald green.   We also ran the Stein's method - this was completely negative (even on the purchased controls).  Any thoughts and/or feedback is appreciated!
> Thanks!
> Kristie
> 
> Christina Thurby, MLT(ASCP), HT, QIHC
> Research Scientist I
> Bristol Myers Squibb
> 812-307-2093
> 
> 
> 
> 
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