[Histonet] Re: Clostridium difficile - what do you do

[Bob Richmond]

I have no idea - possibly no special precautions - I'd address this
question - about Clostridium difficile - to the right person at the
CDC. They'll know.

While on the subject, how do you pronounce "difficile"? I say
dih-FISS-illy - mostly on the basis of how Mozart pronounced it in his
comic motet "Difficile lectu michi mars" (and never mind what that
means in German).

Bob Richmond
Samurai Pathologist
Knoxville TN
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We are about to embark on a project involving C.diff infected GI tissue.

We have processes in place for PPE, decontamination/ disinfection
etc... but I am looking for any evidence whether or not formalin
fixation or subsequent tissue processing will inactivate the spores.

Also, do you take any special precautions in handling/sectioning the
tissue /slides after paraffin embedding.

I am not coming up with much info with my web searches, so I am asking
for help from any lab that works with C. diff.

Brett M. Connolly, Ph.D.
Imaging Research Fellow
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486