[Histonet] b-cells stain

[Lee & Peggy Wenk]

Acetaldehyde can be used instead of paraldehyde, to make aldehyde fuchsin. 
Substitute 1.5 - 2.0 mL acetaldehyde for every 1.0 mL of paraldehyde. 
Acetaldehyde is usually not a restricted drug, a whole lot cheaper than 
acetaldehyde, and once opened, remains good for much longer than 
paraldehyde, when stored in the refrigerator (at least 18 months instead for 
the 2-3 months for paraldehyde). The aldehyde-fuchsin, once made, will only 
be good for about 3-4 weeks, when stored in the refrig, regardless of 
whether it was made with paraldehyde or acetaldehyde.

The source for the exchange was this article: Gabe, M. 1953. Sur quelques 
applications de la coloration par la fuchsine-paraldehyde. Bull. Micros. 
Appl. Ser. 2, 3: 153-162. One of my students, Yelena Mushkina, HTL(ASCP), 
translated it from the French to her native Russian, and then from Russian 
to English. Took a couple of dictionaries! I wrote this up in a Letter to 
the Editor in the Dec. 1996 NSH Journal of Histotechnology "Acetaldehyde as 
a Substitute for Paraldehyde". In case you need some references.

IHC is actually better for demonstrating beta cells - whether for pituitary 
or for pancreas. Just something else to think about.

Peggy A. Wenk, HTL(ASCP)SLS
Beaumont Hospital
Royal Oak, MI 48073


-----Original Message----- 
From: Silvina Molinuevo
Sent: Thursday, December 01, 2011 9:25 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] b-cells stain

Hi Histonetters!!
I want to do Chrome Hematoxylin-Phloxin (from Gomori) to show b-cells. Does 
anybody know if the stain go well with ferrum hematoxylin instead of chrome 
hematoxylin?
I know that the best stain is Aldehyde-fuchsin but I can't import 
paraldehyde because of legal restrictions about that product in my country.
Thanks in advance.

Dr María Silvina Molinuevo
Grupo de Investigacion en
Osteopatias y Metabolismo Mineral
Departamento de Ciencias Biologicas
Facultad de Ciencias Exactas
Universidad Nacional de La Plata
47 y 115
(1900)La Plata
Argentina
www.biol.unlp.edu.ar/giomm
e-mail: silvina.molinuevo <@t> bigfoot.com
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