[Histonet] b-cells stain

[Geoff McAuliffe]

Greetings Silvina:

You do not have to use paraldehyde, you can make a perfectly good 
aldehyde fuchsin using acetaldehyde since one molecule of paraldehyde 
depolymerizes into 3 molecules of acetaldehyde. Just use 3x the amount 
of acetaldehyde. n-butyraldehyde will also work for b-cells. See Buehner 
et al. J.Histochem. Cytochem. 27(3):782787, 1979 for more thorough 
discussion of the chemistry.

And, if a ferrous hematoxylin worked for b-cells we would not need the 
chrome alum variety.

Geoff

On 12/1/2011 9:25 AM, Silvina Molinuevo wrote:
> Hi Histonetters!!
> I want to do Chrome Hematoxylin-Phloxin (from Gomori) to show b-cells. Does anybody know if the stain go well with ferrum hematoxylin instead of chrome hematoxylin?
> I know that the best stain is Aldehyde-fuchsin but I can't import paraldehyde because of legal restrictions about that product in my country.
> Thanks in advance.
>   
> Dr María Silvina Molinuevo
> Grupo de Investigacion en
> Osteopatias y Metabolismo Mineral
> Departamento de Ciencias Biologicas
> Facultad de Ciencias Exactas
> Universidad Nacional de La Plata
> 47 y 115
> (1900)La Plata
> Argentina
> www.biol.unlp.edu.ar/giomm
> e-mail: silvina.molinuevo <@t> bigfoot.com
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>


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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583
mcauliff <@t> umdnj.edu
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