[Histonet] Common Fixatives

[Mehlika Faire]

Depending on the experiment.  Our lab uses 4% PFA for cryosections, Methanol:DMSO (4:1) for whole mount IHC, acetone/4%PFA for cell fixation
-Mehlika

> Date: Wed, 31 Aug 2011 07:45:47 -0700
> From: rjbuesa <@t> yahoo.com
> To: histonet <@t> lists.utsouthwestern.edu; thomas.crowell <@t> novartis.com
> Subject: Re: [Histonet] Common Fixatives
> CC: 
> 
> Do not sweat, 85% = neutral buffered formalin. The remaining 15% are about 20 other used in such a small number of labs that do not merit consideration.
> René J.
> 
> --- On Wed, 8/31/11, Crowell, Thomas <thomas.crowell <@t> novartis.com> wrote:
> 
> 
> From: Crowell, Thomas <thomas.crowell <@t> novartis.com>
> Subject: [Histonet] Common Fixatives
> To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
> Date: Wednesday, August 31, 2011, 9:44 AM
> 
> 
> Hi All,
> 
> I would like to get a list of the most common fixatives that are being used in clinical laboratories, both in the USA as well as globally.  This would be for all types of specimens that come through your labs.
> 
> Any help would be greatly appreciated!
> Thanks,
> Tom
> 
> 
> Thomas Crowell
> Fellow (Molecular Discovery)
> Novartis Institutes for BioMedical
> Research, Inc.
> 45 Sidney Street
> Cambridge, MA 02139
> USA
> 
> Phone    +1  617 8717460
> Fax         +1  N.A.
> thomas.crowell <@t> novartis.com<mailto:thomas.crowell <@t> novartis.com>
> www.novartis.com<http://www.novartis.com/>
> 
> 
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