[Histonet] lot to lot

[Galbraith, Joe]

Mary:  I agree with Rene with one caveat.  The block should be one that you have well characterized over multiple runs with the previous lot # (like a tonsil control).  While not mandatory, I would not use a different or new block for which you have little information.  I prefer to cut successive cuts from the block staining one with the old lot and the other with the new lot.  This may be a bit anal but it seems to work best in our hands.   Joe

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Monday, August 29, 2011 11:21 AM
To: histonet <@t> lists.utsouthwestern.edu; mary.helie <@t> yale.edu
Subject: Re: [Histonet] lot to lot

I think yes!
René J.

--- On Mon, 8/29/11, Mary Helie <mary.helie <@t> yale.edu> wrote:


From: Mary Helie <mary.helie <@t> yale.edu>
Subject: [Histonet] lot to lot
To: histonet <@t> lists.utsouthwestern.edu
Date: Monday, August 29, 2011, 12:06 PM


Good Afternoon

I would like to get an idea of how many slides people are running for lot to lot antibody and detection testing. For example would a tonsil with good internal negative and positive elements be good enough to validate a new lot of LCA?

Thank you
Mary

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