[Histonet] troubleshooting Oil Red O

Geoff McAuliffe mcauliff <@t> umdnj.edu
Mon Aug 29 10:23:14 CDT 2011

It may be that Vector has changed the formulation for Hard Set. I 
suggest you call them up. I have found their technical support to be 


On 8/29/2011 10:30 AM, Denise G Crowley wrote:
> Hi all,
> I'm using the Churukian method  posted by Gayle Callis a few years ago and I have encountered a strange problem.  The lipid stains fine, and the Gill's hematoxylin looks good before I coverslip with Vectashield Hard Set aqueous mounting medium.  But within a matter of minutes, the hematoxylin leaches out.  I popped off the coverslip and tried again with Mayer's hematoxylin with the same results.  I have used Vectashield Hard Set before, in fact I pulled a 5 year old control that still looks great.  Does anyone have any ideas?
> Denise Crowley
> Histology Facility Manager
> Koch Center for Integrative Cancer Research
> Massachusetts Institute of Technology
> 500 Main St. 76-182
> Cambridge MA 02139
> 617-258-8183
> dencrowl <@t> mit.edu
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583
mcauliff <@t> umdnj.edu

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