[Histonet] troubleshooting Oil Red O

Drew Meyer 41dmb41 <@t> gmail.com
Mon Aug 29 09:42:39 CDT 2011

Could it be a bad lot of the Hard Set?


On Mon, Aug 29, 2011 at 10:30, Denise G Crowley <dencrowl <@t> mit.edu> wrote:

> Hi all,
> I'm using the Churukian method  posted by Gayle Callis a few years ago and
> I have encountered a strange problem.  The lipid stains fine, and the Gill's
> hematoxylin looks good before I coverslip with Vectashield Hard Set aqueous
> mounting medium.  But within a matter of minutes, the hematoxylin leaches
> out.  I popped off the coverslip and tried again with Mayer's hematoxylin
> with the same results.  I have used Vectashield Hard Set before, in fact I
> pulled a 5 year old control that still looks great.  Does anyone have any
> ideas?
> Denise Crowley
> Histology Facility Manager
> Koch Center for Integrative Cancer Research
> Massachusetts Institute of Technology
> 500 Main St. 76-182
> Cambridge MA 02139
> 617-258-8183
> dencrowl <@t> mit.edu
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