[Histonet] Does anyone know of a good protocol for pERK staining in PFA fixed tissues?

[mehlika faire]

Hello All,

I was wondering if anyone has a good protocol for pERK staining in PFA fixed tissues.  I have tried several protocols (including Antigen retrieval using trypsin and dnase) but have been unsuccessful so far.  I am trying to identify pERK in 10uM sections that have been fixed in 4% PFA for 1 hour.  If anyone has any insight, I would really appreciate it!

Thanks in advance!

Best,

Mehlika