[Histonet] Does anyone know of a good protocol for pERK staining in
PFA fixed tissues?
mehlikafaire <@t> hotmail.com
Fri Aug 26 17:46:04 CDT 2011
I was wondering if anyone has a good protocol for pERK staining in PFA fixed tissues. I have tried several protocols (including Antigen retrieval using trypsin and dnase) but have been unsuccessful so far. I am trying to identify pERK in 10uM sections that have been fixed in 4% PFA for 1 hour. If anyone has any insight, I would really appreciate it!
Thanks in advance!
More information about the Histonet