[Histonet] RE: Nile Red stain on Froz. muscle bx's

[Houston, Ronald]

J Histochem Cytochem. 1987 May;35(5):619-21.
Application of nile blue and nile red, two fluorescent probes, for detection of lipid droplets in human skeletal muscle.
Bonilla E, Prelle A.
Abstract
Using frozen sections from human muscle biopsies, we assessed the value of Nile blue and Nile red, two fluorescent probes, as stains for lipid droplets in normal and pathological skeletal muscle fibers. In normal muscle, lipid storage disorders, and mitochondrial myopathies, Nile blue stained the lipid droplets as yellow-gold fluorescent structures. The lipid droplets were also seen as yellow-gold fluorescent structures in Nile red-stained sections, but the outstanding feature in these preparations was the staining of the membrane network of the muscle fibers and membrane proliferations in pathological muscle as red-orange fluorescent structures. These results suggest that both Nile blue and Nile red stains are useful for visualization of lipid droplets and membrane proliferations in pathological muscle biopsies.

PMID:3559182[PubMed - indexed for MEDLINE]



Ronnie Houston
Anatomic Pathology Manager
Nationwide Children's Hospital
Columbus OH 43205
(614) 722 5450
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sharon Allen
Sent: Tuesday, August 23, 2011 10:11 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Nile Red stain on Froz. muscle bx's

Hi,
Does anyone have a method for staining frozen skeletal muscle bx's with Nile Red &/or Nile Blue for the detection of lipid droplets.
Our Neuropathologist saw the stain at a conference & would like us to try it. 
Thanks

Sharon Allen
Senior Technologist
Neuropathology Lab MS435U
Health Sciences Centre
825 Sherbrook St.
Winnipeg, Manitoba R3A 1R9
Ph# 787-4615


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