[Histonet] MMA

Reuel Cornelia Reuel.Cornelia <@t> tsrh.org
Thu Aug 18 14:06:15 CDT 2011

Can undecalcified bone MMA embedded tissue be use for Electron Microscopy.The tissue was fixed in formalin dehydrated in grades of alcohol, clear in xylene, infiltrated and embedded in MMA (MMA, dibutyl phthalate,perakdox).  If not,can anyone have a procedure how to prepare tissue that are embedded in MMA for EM. Is this the same procedure done on a paraffin embedded tissue where you melt the paraffin with xylene then hydrate, wash in distilled water then transfer in osmium ,wash in water, dehydrate, PO,resin. 
Thank you for your help.

More information about the Histonet mailing list