[Histonet] Frozen Tissue Protocol

Sheila Fonner sfonner <@t> labpath.com
Wed Aug 17 09:45:18 CDT 2011

Hi everyone,

Yesterday I asked for help with modifying my protocols to stain HSVI, HSVII,
and Zoster.  I appreciate everyone's help.  Now I am back for more info.  I
deleted the depar. and cell conditioning steps and I got specific staining
with little background.  There was just one problem.the pathologist says
that the leukocytes are reacting with the alk. phos on all of the slides.
Does anyone know if a blocking step would help in this case?  Just a
reminder - I am using the Ventana Ultra platform with Ultraview Red
Detection.  Thanks again Histoland.  You never let me down! J



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