[Histonet] RE: H&E on Frozen

[Emily Sours]

I concur on this.
If my boss will let me, I can send you our elaborate protocol for H&E
staining,  It involves many dishes and many washes.  But your H&E will be
perfect.
I don't have the protocol with me right now,as I'm at home but tomorrow I
will ask about sending you the protocol.  I don't see why we couldn't send
you ours, but I'm going to ask just in case.

A great book should leave you with many experiences, and slightly exhausted.
You should live several lives while reading it.
-William Styron



On Tue, Aug 16, 2011 at 2:53 PM, Rathborne, Toni <
trathborne <@t> somerset-healthcare.com> wrote:

> Our protocol is to:
>
> Use a positively charged slide
> Fix in 10% formalin for 1 minute
> Rinse in tap (a few dips)
> Gill 3 for 1 minute
> Rinse in tap (a few dips)
> 3-5 dips in bluing
> Rinse in tap (a few dips)
> Eosin 2-3 dips
> 100% ETOH (a few dips)
> 100% ETOH (a few dips)
> Xylene (dip until clear)
> Mount with Richard- Allan Mounting Medium
>
> After the Gill3, Eosin, and last alcohol, the back of the slide is wiped
> clean of excess solution to prevent carryover.
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:
> histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Reynolds,Donna M
> Sent: Tuesday, August 16, 2011 10:13 AM
> To: 'histonet <@t> lists.utsouthwestern.edu'
> Subject: [Histonet] H&E on Frozen
>
> We do this frequently.
> Fix slides in Methanol (45ml) + formaldehyde (5ml) for 10 minutes.
> Wash several changes of tap water
> Stain with Harris Hematoxylin for 3 minutes Proceed as you would for
> paraffin  sections Get great staining.
> I am sure if you are using a different Hematoxylin you could use it the
> same way you use it for paraffin
>
> Donna Reynolds HT(ASCP)
> Chief Histology Technician, Core Immuno Lab U. T. M.D. Anderson Cancer
> Center, Houston, TX
> 713-792-8106
> e-mail dreynold <@t> mdanderson.org
>
> Message: 2
> Date: Fri, 12 Aug 2011 08:52:11 -0700 (PDT)
> From: Jennifer Sipes <jengirl1014 <@t> yahoo.com>
> Subject: [Histonet] H&E for Frozen Tissue
> To: "histonet <@t> lists.utsouthwestern.edu"
>        <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
>        <1313164331.88113.YahooMailNeo <@t> web125406.mail.ne1.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> Does anyone have a good protocol for staining frozen sections with H&E??
> Also, any mounting medium suggestions?? This is the first time I've been
> asked to do this for frozen.
> ?
> Thanks a bunch everyone!
> Jen
> ?
> Jennifer K. Sipes, ALAT
> Sr. Laboratory Technician
> Johns Hopkins University
> Ross 933
> 720 Rutland Avenue
> Baltimore, MD? 21205
> phone:???? 410-614-0131
> fax:???????? 410-955-9677
> cell:???????? 443-631-6361
> e-mail:? jsipes1 <@t> jhmi.edu
>
> ------------------------------
>
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