[Histonet] Peloris Rapid tissue processor

[Rene J Buesa]

Dear Colleagues:
I have just received the following e-mail from Leica Microsystems.
It is evident that I was wrong and that now the tissues in the Peloris instrument go directly from the 2-propanol to the parafin wax and are not air dried.
I just wanted to share this correction with you.
René J. 

--- On Mon, 8/15/11, Mary.Cheles <@t> leica-microsystems.com <Mary.Cheles <@t> leica-microsystems.com> wrote:


From: Mary.Cheles <@t> leica-microsystems.com <Mary.Cheles <@t> leica-microsystems.com>
Subject: Re: [Histonet] Peloris Rapid tissue processor
To: "Rene J Buesa" <rjbuesa <@t> yahoo.com>
Date: Monday, August 15, 2011, 7:03 PM


Dear Rene,

I read your statement on Peloris today. It is certainly not our intent to
try to censor the information placed on Histonet. However, I would like to
point out that there is some inaccuracy in your assessment.

The  inaccuracy is in the statement that tissues are subjected to "DRY HOT
evaporation.....". This is simply not true. Within the xylene-free
protocols of the instrument, isopropyl does act as a clearant instead of
xylene. Therefore, tissue transitions from isopropyl directly into
paraffin. The temperature of the 1st two paraffins are elevated to
"evaporate" the isopropyl but the tissue is physically in paraffin
undergoing infiltration during this simultaneous process.

If you have any questions, we would be happy to answer them. I am hopeful
that you will see fit to write in to Histonet with a clarifying statement.

We would be happy to send you white papers on the Peloris technology if you
are interested in receiving them.

Best Regards,

Mary Cheles, MPH, HTL, DLM (ASCP)
Director, Technical Services & Education Programs
Leica Microsystems
Biosystems Division
1700 Leider Lane
Buffalo Grove, IL 60089
847.317.5916 Office
847.236.3058 Fax
www.leica-microsystems.com



                                                                           
             Rene J Buesa                                                  
             <rjbuesa <@t> yahoo.co                                             
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             ern.edu                   Histology <@t> skm.org.pk                
                                                                   Subject 
                                       Re: [Histonet] Peloris Rapid tissue 
             08/13/2011 09:19          processor                           
             AM                                                            
                                                                           
                                                                           
                                                                           
                                                                           
                                                                           




Muhammad:
The only thing I dislike about the Peloris technology (it is a technology
in itself) is that after the dehydration with 2-propanol, the tissues are
subjected to a DRY HOT evaporation of the 2-propanol in vacuum before the
infiltration step with melted paraffin.
That step  of drying out the tissues to eliminate the 2-propanol to
"facilitate" the infiltration is the one I do not like because the tissues
are exposed to a very high gradient.
If you end buying the instrument I think you should run a large series of
validation tests to find out if the results you obtain with the Peloris
compare with what you are used to, not referring to the sectioning quality
of the blocks, but to their microscopic appearance..
Peloris was developed in Australia by VisionBioSystems and later bought by
Leica Microsystems.
Although there were tests published by VisionBioSystems, all referred to
animal tissues, and the instrument was never independently validated. Leica
did not make known (published) independent validations.
I for one would never subject the tissues to a hot dry desiccation before
infiltration.
I hope this will help you in your decision.
René J.
--- On Sat, 8/13/11, tahseen <@t> brain.net.pk <tahseen <@t> brain.net.pk> wrote:


From: tahseen <@t> brain.net.pk <tahseen <@t> brain.net.pk>
Subject: [Histonet] Peloris Rapid tissue processor
To: histonet <@t> lists.utsouthwestern.edu
Cc: Histology <@t> skm.org.pk
Date: Saturday, August 13, 2011, 6:18 AM



Dear All,
Our lab is in the process of purchasing our  automated tissue processor
(Peloris Rapid tissue processor).
I would really appreciate comments from anyone who really likes, or
dislikes the processor that they are using.

        Thanks in advance!!!

        Muhammad Tahseen
        Histology Supervisor
         Deptt. Pathology
        Shaukat Khanum Cancer Hospital
        And Research Center Lahore. Pakistan
        Ph.   +92 42 5180725-36 Ext 2369,
        Fax. +92 42  5180723
        e-mail. Histology <@t> skm.org.pk



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