[Histonet] protocol for in situ RT_PCR

[Adam .]

I've used 10% zinc buffered formalin from Anatech (no alcohol) on mouse
bones with good results. It's possible that the alcohol is denaturing your
antigens of interest. Is there a reason you need to use alcoholic formalin?

Adam

On Tue, Aug 16, 2011 at 7:45 AM, Liang, Frank <FLiang <@t> wellstatoc.com> wrote:

> Hello,
>
> Does anyone have used alcoholic zinc-formalin to fix mouse tissues? We
> tried  a few times with this fixative for immunohistochemical staining, it
> did not work, but when switched back to 4% paraformaldehyde, the
> immunostaining worked. We like alcoholic zinc-formalin as it gives better
> morphology. Any suggestions?
>
> Thanks,
>
> Frank
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:
> histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Louise Renton
> Sent: Tuesday, August 16, 2011 4:17 AM
> To: Barone, Carol; Histonet
> Subject: Re: [Histonet] protocol for in situ RT_PCR
>
> hi could you add me on too pelase?
>
> On Mon, Aug 15, 2011 at 11:09 PM, Barone, Carol <cbarone <@t> nemours.org>
> wrote:
>
> > Anyone got a hot protocol for In situ RT-PCR? Haven't done one in 5
> > years (on my Perk and Elmer)...Know there must be some great new
> > reagents and kits out there...opinions? Send to cbarone <@t> nemours.org. Thx
> > CB
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>
>
>
> --
> Louise Renton
> Bone Research Unit
> University of the Witwatersrand
> Johannesburg
> South Africa
> +27 11 717 2298 (tel & fax)
> 073 5574456 (emergencies only)
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> George Carlin
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