[Histonet] protocol for in situ RT_PCR

[Liang, Frank]

Hello, 

Does anyone have used alcoholic zinc-formalin to fix mouse tissues? We tried  a few times with this fixative for immunohistochemical staining, it did not work, but when switched back to 4% paraformaldehyde, the immunostaining worked. We like alcoholic zinc-formalin as it gives better morphology. Any suggestions?

Thanks,

Frank  

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Louise Renton
Sent: Tuesday, August 16, 2011 4:17 AM
To: Barone, Carol; Histonet
Subject: Re: [Histonet] protocol for in situ RT_PCR

hi could you add me on too pelase?

On Mon, Aug 15, 2011 at 11:09 PM, Barone, Carol <cbarone <@t> nemours.org> wrote:

> Anyone got a hot protocol for In situ RT-PCR? Haven't done one in 5
> years (on my Perk and Elmer)...Know there must be some great new
> reagents and kits out there...opinions? Send to cbarone <@t> nemours.org. Thx
> CB
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>



-- 
Louise Renton
Bone Research Unit
University of the Witwatersrand
Johannesburg
South Africa
+27 11 717 2298 (tel & fax)
073 5574456 (emergencies only)
"There are nights when the wolves are silent and only the moon howls".
George Carlin
No trees were killed in the sending of this message.
However, many electrons were terribly inconvenienced.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet