[Histonet] Peloris Rapid tissue processor
Rene J Buesa
rjbuesa <@t> yahoo.com
Sat Aug 13 09:19:34 CDT 2011
The only thing I dislike about the Peloris technology (it is a technology in itself) is that after the dehydration with 2-propanol, the tissues are subjected to a DRY HOT evaporation of the 2-propanol in vacuum before the infiltration step with melted paraffin.
That step of drying out the tissues to eliminate the 2-propanol to "facilitate" the infiltration is the one I do not like because the tissues are exposed to a very high gradient.
If you end buying the instrument I think you should run a large series of validation tests to find out if the results you obtain with the Peloris compare with what you are used to, not referring to the sectioning quality of the blocks, but to their microscopic appearance..
Peloris was developed in Australia by VisionBioSystems and later bought by Leica Microsystems.
Although there were tests published by VisionBioSystems, all referred to animal tissues, and the instrument was never independently validated. Leica did not make known (published) independent validations.
I for one would never subject the tissues to a hot dry desiccation before infiltration.
I hope this will help you in your decision.
--- On Sat, 8/13/11, tahseen <@t> brain.net.pk <tahseen <@t> brain.net.pk> wrote:
From: tahseen <@t> brain.net.pk <tahseen <@t> brain.net.pk>
Subject: [Histonet] Peloris Rapid tissue processor
To: histonet <@t> lists.utsouthwestern.edu
Cc: Histology <@t> skm.org.pk
Date: Saturday, August 13, 2011, 6:18 AM
Our lab is in the process of purchasing our automated tissue processor
(Peloris Rapid tissue processor).
I would really appreciate comments from anyone who really likes, or
dislikes the processor that they are using.
Thanks in advance!!!
Shaukat Khanum Cancer Hospital
And Research Center Lahore. Pakistan
Ph. +92 42 5180725-36 Ext 2369,
Fax. +92 42 5180723
e-mail. Histology <@t> skm.org.pk
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