[Histonet] RE: p16 on the Bond
McCabe, Sara J.
SJMccabe <@t> drmc.org
Fri Aug 12 14:32:22 CDT 2011
Hi Nancy,
We too use p16 on the Bond Max and Bond III using Leica ER 1 for 20 min. It is working great for us!
Sara McCabe, HT (ASCP)
DuBois Regional Medical Center
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Sent: Monday, July 18, 2011 1:02 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 92, Issue 23
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Today's Topics:
1. Invitation to connect on LinkedIn (Sheree Holmes via LinkedIn)
2. Water droplets on slides (Esther Peters)
3. RNAlater buffer & fixation/embedding in OCT (Megan French)
4. positive staining on my secondary only controls...need a DAB
superquench? (Megan French)
5. Lab humidity range (L White)
6. P16 on the BOND (Nancy Schmitt)
7. Re: Histobath revised (Johnson, Teri)
8. Alcian blue-safranin (Figliuolo, Leticia)
9. RE: Re: Histobath revised (Houston, Ronald)
10. RE: positive staining on my secondary only controls...need a
DAB superquench? (Elizabeth Chlipala)
11. RE: P16 on the BOND (Settembre, Dana)
12. Spirabrush (Gauch, Vicki)
13. Leica SELECTECH (Breeden, Sara)
14. acceptance criteria of IHC stains (Elizabeth Chlipala)
15. Peggy Wenk's job postings (Lee & Peggy Wenk)
----------------------------------------------------------------------
Message: 1
Date: Sun, 17 Jul 2011 22:10:33 +0000 (UTC)
From: Sheree Holmes via LinkedIn <member <@t> linkedin.com>
Subject: [Histonet] Invitation to connect on LinkedIn
To: David Edmondson <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<1217399822.5174163.1310940633806.JavaMail.app <@t> ela4-bed81.prod>
Content-Type: text/plain; charset=UTF-8
LinkedIn
------------
Sheree Holmes requested to add you as a connection on LinkedIn:
------------------------------------------
David,
I'd like to add you to my professional network on LinkedIn.
- Sheree
Accept invitation from Sheree Holmes
http://www.linkedin.com/e/yvpgd1-gq8k7etn-f/qXtGZ0-QiF70UPNqEunZRx9zbUTaXy-_ifnGa0-b4uheRh4MMF/blk/I133508382_13/1BpC5vrmRLoRZcjkkZt5YCpnlOt3RApnhMpmdzgmhxrSNBszYPclYOe3cUc3kPcP59bT9NtPlhgCBAbPgTc3cNdPcSdjcLrCBxbOYWrSlI/EML_comm_afe/
View invitation from Sheree Holmes
http://www.linkedin.com/e/yvpgd1-gq8k7etn-f/qXtGZ0-QiF70UPNqEunZRx9zbUTaXy-_ifnGa0-b4uheRh4MMF/blk/I133508382_13/3cNnP8UcPwMdjcPckALqnpPbOYWrSlI/svi/
------------------------------------------
DID YOU KNOW your LinkedIn profile helps you control your public image when people search for you? Setting your profile as public means your LinkedIn profile will come up when people enter your name in leading search engines. Take control of your image!
http://www.linkedin.com/e/yvpgd1-gq8k7etn-f/ewp/inv-22/
--
(c) 2011, LinkedIn Corporation
------------------------------
Message: 2
Date: Sun, 17 Jul 2011 19:10:36 -0400
From: Esther Peters <epeters2 <@t> gmu.edu>
Subject: [Histonet] Water droplets on slides
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID: <4E236BEC.90509 <@t> gmu.edu>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed
I am having problems with water droplets appearing on slides when
transferring the rack from the last 100% dehydrant (Richard-Allan) to
either SafeClear II or xylenes. I saw that some of you had noted this
problem might be in the xylene substitute, but switching to xylene
seemed to solve it. I am wondering if the "100%" isn't? I have poured
immediately before use from freshly opened bottles, and still have the
problem. Would appreciate any help (of course, the humidity is high
right now, but...)!
--
Esther C. Peters, Ph.D., Assistant Professor; Department of
Environmental Science & Policy; George Mason University
------------------------------
Message: 3
Date: Mon, 18 Jul 2011 13:43:25 +1000
From: "Megan French" <megan.french <@t> mcri.edu.au>
Subject: [Histonet] RNAlater buffer & fixation/embedding in OCT
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <DE098B84182F9B4F966EE97D926888D9B48B33 <@t> murmx.mcri.edu.au>
Content-Type: text/plain; charset="us-ascii"
Has anyone successful fixed & embedded tissues in OCT after using
RNAlater buffer? I've found a paper that says this doesn't affect tissue
integrity but I need for info regarding protocol (To defrost? Fixative?
Timing? Temperature Etc) ...and more than anything feedback from someone
who has used/uses this product.
Product: http://www.ambion.com/techlib/resources/RNAlater/
Paper: http://www.nature.com/modpathol/journal/v14/n2/pdf/3880267a.pdf
Miss Megan French BBiomedSc(Deakin); BSc(Hons)(Melbourne)
Research Assistant
Surgical Research; Infection & Immunity Theme
Murdoch Children's Research Institute
The Royal Children's Hospital
Flemington Road Parkville Victoria 3052 Australia
E megan.french <@t> mcri.edu.au
*
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Message: 4
Date: Mon, 18 Jul 2011 13:44:55 +1000
From: "Megan French" <megan.french <@t> mcri.edu.au>
Subject: [Histonet] positive staining on my secondary only
controls...need a DAB superquench?
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <DE098B84182F9B4F966EE97D926888D9B48B34 <@t> murmx.mcri.edu.au>
Content-Type: text/plain; charset="us-ascii"
Having some trouble with my IHC optimisation. Hopefully it's a simple
fix someone may be able to provide some guidance (Im a junior RA! Hello
histoworld!).
Im using a DAB protocol with swine-anti-rabbit HRP on human colon. I
have successful staining of my primary (yay!), somewhat successful
staining with my antibody preabsorbed with peptide (yet still some
staining)...BUT im seeing positive staining on my secondary only
controls (may explain why im getting a bit of staining on my preabsorped
specimens). I quench with 5% hydrogen peroxide for 5 min on day 1 (prior
to primaries/no primary if secondary control) and on day 2 add 1 DAKO
DAB chromogen tablet to 10ml PBS and 10ul hydrogen peroxide again before
immersing slides for 8 min, then stain with haematoxylin per usual,
dehydrate, clear & mount. I feel like I need a super quencher or
something?! Any tips would be most helpful. (My slides also undergo
antigen retrieval in citrate buffer ph6 for 10min @ 95deg and 20min at
RT)
Miss Megan French BBiomedSc(Deakin); BSc(Hons)(Melbourne)
Research Assistant
Surgical Research; Infection & Immunity Theme
Murdoch Children's Research Institute
The Royal Children's Hospital
Flemington Road Parkville Victoria 3052 Australia
E megan.french <@t> mcri.edu.au
*
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------------------------------
Message: 5
Date: Mon, 18 Jul 2011 06:27:00 -0400
From: L White <lori.w <@t> sympatico.ca>
Subject: [Histonet] Lab humidity range
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <BLU0-SMTP79DA6CA75B1890C6B533B4F04A0 <@t> phx.gbl>
Content-Type: text/plain; charset="us-ascii"
Sakura has developed a threshold for humidity if you are using their film
coverslipper. Other than that, I am not aware of any specific requirements
for routine histology equipment.
Lori
------------------------------
Message: 6
Date: Mon, 18 Jul 2011 13:24:28 +0000
From: Nancy Schmitt <Nancy_Schmitt <@t> pa-ucl.com>
Subject: [Histonet] P16 on the BOND
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<906B4DA90ED1DB4DB6C7E94D7CEE6C36791319 <@t> PEITHA.wad.pa-ucl.com>
Content-Type: text/plain; charset="us-ascii"
Happy Monday-
I should have been more specific in my question - is anyone using p16 on the BOND IHC instrument?
Thanks
Nancy
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Message: 7
Date: Mon, 18 Jul 2011 08:44:24 -0500
From: "Johnson, Teri" <TJJ <@t> stowers.org>
Subject: [Histonet] Re: Histobath revised
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<2C40E43D1F7A56408C4463FD245DDDF977056669 <@t> EXCHMB-02.stowers-institute.org>
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Dr. Richmond recommended using non-flammable and non-explosive Novec (tm) Engineered Fluid HFE-7100. We are currently using this for our freezing and it works very well. The main thing we have noticed is the blocks float in it and do not sink as they would with other solvents.
It is a 3M product, and my contact there last year was ebinder <@t> mmm.com (Erin Binder).
Teri Johnson, HT(ASCP)QIHC
Head, Histology and Electron Microscopy
Stowers Institute for Medical Research
Kansas City, MO
------------------------------
Message: 8
Date: Mon, 18 Jul 2011 09:44:39 -0400
From: "Figliuolo, Leticia" <leticia.figliuolo <@t> roche.com>
Subject: [Histonet] Alcian blue-safranin
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<069E6CF048B915488720412DAFD1474D03FC377975 <@t> RNUMSEM702.nala.roche.com>
Content-Type: text/plain; charset="us-ascii"
Hello everybody,
I was wondering if anyone has a protocol for Alcian Blue-Safranin (for mast cells).
I would greatly appreciate any help.
Thank you!
Leticia Figliuolo
e-mail: leticia.figliuolo <@t> roche.com
------------------------------
Message: 9
Date: Mon, 18 Jul 2011 13:57:38 +0000
From: "Houston, Ronald" <Ronald.Houston <@t> nationwidechildrens.org>
Subject: [Histonet] RE: Re: Histobath revised
To: "'Johnson, Teri'" <TJJ <@t> stowers.org>,
"'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<E5D0CD2352E46545A0C0EBE308CCCE530BAC7B <@t> L1PERDWXMB01.childrensroot.net>
Content-Type: text/plain; charset="us-ascii"
I second Terri's comments
Ronnie Houston
Anatomic Pathology Manager
Nationwide Children's Hospital
Columbus OH 43205
(614) 722 5450
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Johnson, Teri
Sent: Monday, July 18, 2011 9:44 AM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] Re: Histobath revised
Dr. Richmond recommended using non-flammable and non-explosive Novec (tm) Engineered Fluid HFE-7100. We are currently using this for our freezing and it works very well. The main thing we have noticed is the blocks float in it and do not sink as they would with other solvents.
It is a 3M product, and my contact there last year was ebinder <@t> mmm.com (Erin Binder).
Teri Johnson, HT(ASCP)QIHC
Head, Histology and Electron Microscopy
Stowers Institute for Medical Research
Kansas City, MO
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Message: 10
Date: Mon, 18 Jul 2011 08:32:44 -0600
From: Elizabeth Chlipala <liz <@t> premierlab.com>
Subject: RE: [Histonet] positive staining on my secondary only
controls...need a DAB superquench?
To: 'Megan French' <megan.french <@t> mcri.edu.au>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<14E2C6176416974295479C64A11CB9AE1DECBA051A <@t> SBS2K8.premierlab.local>
Content-Type: text/plain; charset="us-ascii"
Megan
Pre absorbing your antibody for a control for IHC is not always going to work there are publications out there that do not recommend it. I'll try to find the reference I'm talking about. I pulled this out of one of my presentations
Protocols available on the web - for western blots
http://www.upstate.com/misc/protocol_detail.q.prot.e.peptide-competion.a.name.e.Peptide_Competition
http://store.crpinc.com/prot_peptide_comp.aspx
"The absorption control is less important because if cannot determine whether the protein bound in the tissue is the same protein that used for absorption. Therefore, the recommended guidelines for immunohistochemistry include negative controls and positive controls but do not include absorption controls."
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308-1592
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
www.premierlab.com
Ship to address:
1567 Skyway Drive, Unit E
Longmont, CO 80504
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Megan French
Sent: Sunday, July 17, 2011 9:45 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] positive staining on my secondary only controls...need a DAB superquench?
Having some trouble with my IHC optimisation. Hopefully it's a simple
fix someone may be able to provide some guidance (Im a junior RA! Hello
histoworld!).
Im using a DAB protocol with swine-anti-rabbit HRP on human colon. I
have successful staining of my primary (yay!), somewhat successful
staining with my antibody preabsorbed with peptide (yet still some
staining)...BUT im seeing positive staining on my secondary only
controls (may explain why im getting a bit of staining on my preabsorped
specimens). I quench with 5% hydrogen peroxide for 5 min on day 1 (prior
to primaries/no primary if secondary control) and on day 2 add 1 DAKO
DAB chromogen tablet to 10ml PBS and 10ul hydrogen peroxide again before
immersing slides for 8 min, then stain with haematoxylin per usual,
dehydrate, clear & mount. I feel like I need a super quencher or
something?! Any tips would be most helpful. (My slides also undergo
antigen retrieval in citrate buffer ph6 for 10min @ 95deg and 20min at
RT)
Miss Megan French BBiomedSc(Deakin); BSc(Hons)(Melbourne)
Research Assistant
Surgical Research; Infection & Immunity Theme
Murdoch Children's Research Institute
The Royal Children's Hospital
Flemington Road Parkville Victoria 3052 Australia
E megan.french <@t> mcri.edu.au
*
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------------------------------
Message: 11
Date: Mon, 18 Jul 2011 11:02:37 -0400
From: "Settembre, Dana" <settembr <@t> umdnj.edu>
Subject: [Histonet] RE: P16 on the BOND
To: 'Nancy Schmitt' <Nancy_Schmitt <@t> pa-ucl.com>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<B64B947688FB794A8C191D19F22927C8C4225F12 <@t> UMDEXMBX02.core.umdnj.edu>
Content-Type: text/plain; charset="us-ascii"
Hi Nancy,
I am using the p16 with the Bond.
I am using the Bond's ER1 for 20min with their Refine detection kit.
Dana Settembre
University Hospital - UMDNJ
Newark, NJ
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt
Sent: Monday, July 18, 2011 9:24 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] P16 on the BOND
Happy Monday-
I should have been more specific in my question - is anyone using p16 on the BOND IHC instrument?
Thanks
Nancy
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Message: 12
Date: Mon, 18 Jul 2011 11:16:57 -0400
From: "Gauch, Vicki" <GauchV <@t> mail.amc.edu>
Subject: [Histonet] Spirabrush
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<C5E841FC4B5BA94987B9E68B5396A9AF597DB39909 <@t> MS-EXCH-CCR02.AMCNT.AMC.EDU>
Content-Type: text/plain; charset="us-ascii"
Hi,
I was wondering if anyone has had any experience with processing specimens obtained using the Spirabrush for GYN cases. We have an account who would like to submit his specimens using this method and we wanted to know if people are handling these as Cytology specimens or Histology specimens. Any information would be greatly appreciated.
Thanks,
Vicki Gauch
AMCH
Albany, NY
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Message: 13
Date: Mon, 18 Jul 2011 10:05:18 -0600
From: "Breeden, Sara" <sbreeden <@t> nmda.nmsu.edu>
Subject: [Histonet] Leica SELECTECH
To: <Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<4D14F0FC9316DD41972D5F03C070908B051DF8C1 <@t> nmdamailsvr.nmda.ad.nmsu.edu>
Content-Type: text/plain; charset="us-ascii"
Need to speak with a Technical contact regarding SelecTech staining
system - someone who can give details about expiration dates. Cannot
find contact info online. Thanks!
Sally Breeden, HT(ASCP)
New Mexico Department of Agriculture
Veterinary Diagnostic Services
1101 Camino de Salud NE
Albuquerque, NM 87102
505-383-9278 (Histology Lab)
------------------------------
Message: 14
Date: Mon, 18 Jul 2011 10:14:23 -0600
From: Elizabeth Chlipala <liz <@t> premierlab.com>
Subject: [Histonet] acceptance criteria of IHC stains
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<14E2C6176416974295479C64A11CB9AE1DECBA0524 <@t> SBS2K8.premierlab.local>
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Hello everyone
Has anyone out there had to write acceptance criteria for a USP document, any help would be appreciated.
Thanks in advance
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308-1592
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
www.premierlab.com<http://www.premierlab.com>
Ship to address:
1567 Skyway Drive, Unit E
Longmont, CO 80504
------------------------------
Message: 15
Date: Mon, 18 Jul 2011 12:43:56 -0400
From: "Lee & Peggy Wenk" <lpwenk <@t> sbcglobal.net>
Subject: [Histonet] Peggy Wenk's job postings
To: "Histonet" <Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <033A63EE883E4A36AFBE246D19F2A0DB <@t> HP2010>
Content-Type: text/plain; charset="iso-8859-1"
Histonetters -
There seems to be a little glitch in our hospital's application process. Several people have said that they have applied for the position of Program Director for the Schools of Histotechnology at William Beaumont Hospital, Royal Oak, MI. But no one is showing up in the system.
Could all those interested please go two things:
1. Reapply
2. Send resume (in a separate email, in addition to attaching it to the application process) to our Human Resources person and to me, just in case the application doesn't show up again.
Diane Soper, Human Resources dsoper <@t> beaumont.edu
Peggy Wenk, School, pwenk <@t> beaumont.edu
For more information about the job, and directions on where to apply, go to the NSH website:
www.nsh.org
On the right, click on Career Center
On the left/middle, click on View Jobs
Look for the 6/30/11 Program Director position, click on View
I apologize for the inconvenience.
Peggy A. Wenk, HTL(ASCP)SLS
Program Director
Schools of Histotechnology
William Beaumont Hospital
Royal Oak, MI 48073
Lee & Peggy Wenk
------------------------------
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