[Histonet] RE: controls on patient slide
McCabe, Sara J.
SJMccabe <@t> drmc.org
Fri Aug 12 14:07:23 CDT 2011
We always put our patient and control tissue on the same slide. We have many controls already cut and then we place the patient tissue below. If we are using blanks that were previously cut then we cut the control and place that below the patient tissue. We chose to not invest in the pre-marked slides. After processing we make a box with a red sharpie around the control tissue so there is no confusion as to which is the patient tissue and which is control.
Sara McCabe, HT (ASCP)
DuBois Regional Medical Center
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu [histonet-request <@t> lists.utsouthwestern.edu]
Sent: Thursday, July 21, 2011 1:02 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 92, Issue 28
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Today's Topics:
1. RE: Assisting with Autopsies (Horn,Hazel)
2. Re: Leica Service Technician (Nicole Tatum)
3. RE: Leica Service Technician (Suresch, Donna L.)
4. Research microtomes (Adrienne Anderson)
5. Re: Research microtomes (Jack Ratliff)
6. Re: Research microtomes (Adrienne Anderson)
7. Re: Research microtomes (Jack Ratliff)
8. embalmers using formaldehyde - NYT story (Bob Richmond)
9. Re: Microtome draft shield (Joseph Saby)
10. Re: (Denise Piontek)
11. Re: Research microtomes (Adrienne Anderson)
12. Re: Research microtomes (Jack Ratliff)
13. Small Slide Scanner (Sheila Haas)
14. Fast Blue stabilization (carpj)
----------------------------------------------------------------------
Message: 1
Date: Wed, 20 Jul 2011 12:17:48 -0500
From: "Horn,Hazel" <HornHV <@t> archildrens.org>
Subject: [Histonet] RE: Assisting with Autopsies
To: "'Amy Self'" <ASelf <@t> georgetownhospitalsystem.org>,
"'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<25A4DE08332B19499904459F00AAACB7198B85ACEA <@t> EVS1.archildrens.org>
Content-Type: text/plain; charset="us-ascii"
We have an autopsy assistant and when he's not busy with autopsies (which is often) he works in our histology lab as an assistant!!
Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Autopsy/Histology/Transcription
Arkansas Children's Hospital
1 Children's Way Slot 820
Little Rock, AR 72202
phone 501.364.4240
fax 501.364.3155
visit us on the web at: www.archildrens.org
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Amy Self
Sent: Tuesday, July 19, 2011 2:12 PM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] Assisting with Autopsies
Hello All,
We are small hospital that does approximately 5-10 autopsies a year. This being said our administration department does not want to hire a diener to assist with these autopsies. So I have decided to turn to all of you out there in histoland for a little poll.
Does your facility use histotechs or a diener to assist with the autopsy?
Thanks in advance for all of your help, Amy
Amy Self
Georgetown Hospital System
843-527-7179
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Message: 2
Date: Wed, 20 Jul 2011 14:08:10 -0400 (EDT)
From: "Nicole Tatum" <nicole <@t> dlcjax.com>
Subject: Re: [Histonet] Leica Service Technician
To: "Amanda Madden" <amkmadden <@t> gmail.com>,
histonet <@t> lists.utsouthwestern.edu
Message-ID:
<3934.208.62.167.196.1311185290.squirrel <@t> webmail.realpages.com>
Content-Type: text/plain;charset=iso-8859-1
Amanda,
Im not exactly sure which part of your machine is freezing based on your
description, but, I have a leica 1510 and it has freezing issues. Everyone
I have ever used does this. The bar that hold the specimen chucks ices and
freezes over like crazy.. It will frezze the chuck in the bar holder
solid. I have to get a hammer and beat it sometimes..lol. So, before I use
my machine I wipe 100% alcohol across the bar to de-ice it and the chucks
dont get stuck..Do not use so much that you lower your temp. Also, do not
get on the oct or the stage because your blocks will not cut and be
mush... Just wipe the areas daily with alcohol.. Hope this helps.
Nicole Tatum, HT ASCP
Hi Histonetters!
>
> A few months back I emailed regarding a service contract through Leica for
> our CM 3050S, and (unfortunately?) we chose not to purchase one. This week
> we have had a serious issue with it... the specimen head and arm is
> covered
> in frost, and the object temperature sensor is reading ## instead of a
> temp.
> In any case, we called Leica and asked for a service call, but it is
> extremely expensive and they couldn't give us an estimate of when they
> will
> be here because cryostats used for clinical applications have priority
> over
> those, like ours, that are used for research. Understandable, but
> frustrating nonetheless. So my question is: does anyone know of a good,
> reputable cryostat service technician (who is authorized by leica, if
> possible) that is located in the Boston, MA area?
>
> Any help would be greatly appreciated!
>
> Thanks in advance,
> Amanda
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
------------------------------
Message: 3
Date: Wed, 20 Jul 2011 14:16:40 -0400
From: "Suresch, Donna L." <donna_suresch <@t> merck.com>
Subject: [Histonet] RE: Leica Service Technician
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<2C9A1D9608959940943F357E0A470FF89765A7FB5A <@t> USCTMXP51005.merck.com>
Content-Type: text/plain; charset="us-ascii"
Hello Amanda,
We are located in Eastern Pa and we use Belair Instruments out of NJ (908-518-2662). We have been very happy with their service on our cryostats.
Donna L. Suresch
Merck Research Laboratories
Research Biologist
Imaging Research - West Point
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: Wednesday, July 20, 2011 1:02 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 92, Issue 27
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Today's Topics:
1. Leica Service Technician (Amanda Madden)
2. Controls with patient specimen on same slide (SHargrove <@t> urhcs.org)
3. please remove me from the list, thank you! (Robert Cordero)
4. RE: Leica Service Technician (Sherwood, Margaret )
5. Cat Scratch control (Houston, Ronald)
6. RE: Leica Service Technician (Tom McNemar)
7. RE: Controls with patient specimen on same slide (Tom McNemar)
8. RE: Assisting with Autopsies (Weems, Joyce)
9. Controls with patient specimen on same slide (Nancy Schmitt)
----------------------------------------------------------------------
Message: 1
Date: Wed, 20 Jul 2011 11:32:07 -0400
From: Amanda Madden <amkmadden <@t> gmail.com>
Subject: [Histonet] Leica Service Technician
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<CAC=+zgXtEK9=c0_nUSddHcSp4_7SDsn2jFxT3kYCJh=NSwUH0A <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Hi Histonetters!
A few months back I emailed regarding a service contract through Leica for
our CM 3050S, and (unfortunately?) we chose not to purchase one. This week
we have had a serious issue with it... the specimen head and arm is covered
in frost, and the object temperature sensor is reading ## instead of a temp.
In any case, we called Leica and asked for a service call, but it is
extremely expensive and they couldn't give us an estimate of when they will
be here because cryostats used for clinical applications have priority over
those, like ours, that are used for research. Understandable, but
frustrating nonetheless. So my question is: does anyone know of a good,
reputable cryostat service technician (who is authorized by leica, if
possible) that is located in the Boston, MA area?
Any help would be greatly appreciated!
Thanks in advance,
Amanda
------------------------------
Message: 2
Date: Wed, 20 Jul 2011 10:35:33 -0500
From: SHargrove <@t> urhcs.org
Subject: [Histonet] Controls with patient specimen on same slide
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<OFE0363896.5E031032-ON862578D3.005508B0-862578D3.0055A776 <@t> urhcs.org>
Content-Type: text/plain; charset="us-ascii"
In our Lab we put a control on every slide. We keep multiple controls cut
and stored in refrigerator. Most are multi- tissue controls. We use the
Ventana detection kits and it is not cost efficient to use 2 uses of the
detection kit per antibody. Of course there are times that this will not
work, then our cost per test goes up, but we cannot charge any more for a
control.
(Embedded image moved to file: pic32000.jpg)
------------------------------
Message: 3
Date: Wed, 20 Jul 2011 15:37:44 +0000
From: Robert Cordero <Robert.Cordero <@t> comphealth.com>
Subject: [Histonet] please remove me from the list, thank you!
To: "Histonet <@t> lists.utsouthwestern.edu"
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<0BA36F96367E8A4CBB27E112FAA6488A0679E9 <@t> vslcexmbp02.mychg.com>
Content-Type: text/plain; charset="us-ascii"
------------------------------
Message: 4
Date: Wed, 20 Jul 2011 11:43:46 -0400
From: "Sherwood, Margaret " <MSHERWOOD <@t> PARTNERS.ORG>
Subject: RE: [Histonet] Leica Service Technician
To: "Amanda Madden" <amkmadden <@t> gmail.com>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<073AE2BEA1C2BA4A8837AB6C4B943D9708DB58D3 <@t> PHSXMB30.partners.org>
Content-Type: text/plain; charset="us-ascii"
Amanda,
We have a Microm HM550 cryostat and have used Brian J. Hurley (New England
Biomedical Services). He is an independent service engineer. He's very good.
In fact ThermoFisher uses him if a service visit is needed.
781-331-8642
617-774-7368 (cell)
Peggy
Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (EDR 214)
Massachusetts General Hospital
50 Blossom Street
Boston, MA 02114-2696
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-1206 (fax)
msherwood <@t> partners.org
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Amanda Madden
Sent: Wednesday, July 20, 2011 11:32 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Leica Service Technician
Hi Histonetters!
A few months back I emailed regarding a service contract through Leica for
our CM 3050S, and (unfortunately?) we chose not to purchase one. This week
we have had a serious issue with it... the specimen head and arm is covered
in frost, and the object temperature sensor is reading ## instead of a temp.
In any case, we called Leica and asked for a service call, but it is
extremely expensive and they couldn't give us an estimate of when they will
be here because cryostats used for clinical applications have priority over
those, like ours, that are used for research. Understandable, but
frustrating nonetheless. So my question is: does anyone know of a good,
reputable cryostat service technician (who is authorized by leica, if
possible) that is located in the Boston, MA area?
Any help would be greatly appreciated!
Thanks in advance,
Amanda
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------------------------------
Message: 5
Date: Wed, 20 Jul 2011 15:55:50 +0000
From: "Houston, Ronald" <Ronald.Houston <@t> nationwidechildrens.org>
Subject: [Histonet] Cat Scratch control
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>, "ihcrg <@t> googlegroups.com"
<ihcrg <@t> googlegroups.com>
Message-ID:
<E5D0CD2352E46545A0C0EBE308CCCE530BC6D1 <@t> L1PERDWXMB01.childrensroot.net>
Content-Type: text/plain; charset="us-ascii"
Does anyone have an extra Cat Scratch control block to spare? We currently buy control sections from Newcomer, but I'm not convinced they are that good.
Thanks
Ronnie Houston, MS HT(ASCP)QIHC
Anatomic Pathology Manager
ChildLab, a Division of Nationwide Children's Hospital
www.childlab.com
700 Children's Drive
Columbus, OH 43205
(P) 614-722-5450
(F) 614-722-2899
ronald.houston <@t> nationwidechildrens.org<mailto:ronald.houston <@t> nationwidechildrens.org>
www.NationwideChildrens.org<http://www.NationwideChildrens.org>
"One person with passion is better than forty people merely interested."
~ E.M. Forster
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Message: 6
Date: Wed, 20 Jul 2011 12:26:11 -0400
From: Tom McNemar <TMcNemar <@t> lmhealth.org>
Subject: RE: [Histonet] Leica Service Technician
To: 'Amanda Madden' <amkmadden <@t> gmail.com>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
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<E9A90E28259D2F4E84308C5E8EA8F7B4381967AD20 <@t> lmhs-exchange.lmhealth.org>
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It is really just a freezer with a microtome in it. Do you have an on-site refrigeration guy that could look at it? I have used our in-house guy a time or two. Ours is pretty simple and does not have all of the electronics that you may have on the Leica but it is worth a shot.
Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcnemar <@t> lmhealth.org
www.LMHealth.org
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Amanda Madden
Sent: Wednesday, July 20, 2011 11:32 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Leica Service Technician
Hi Histonetters!
A few months back I emailed regarding a service contract through Leica for
our CM 3050S, and (unfortunately?) we chose not to purchase one. This week
we have had a serious issue with it... the specimen head and arm is covered
in frost, and the object temperature sensor is reading ## instead of a temp.
In any case, we called Leica and asked for a service call, but it is
extremely expensive and they couldn't give us an estimate of when they will
be here because cryostats used for clinical applications have priority over
those, like ours, that are used for research. Understandable, but
frustrating nonetheless. So my question is: does anyone know of a good,
reputable cryostat service technician (who is authorized by leica, if
possible) that is located in the Boston, MA area?
Any help would be greatly appreciated!
Thanks in advance,
Amanda
_______________________________________________
Histonet mailing list
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you.
------------------------------
Message: 7
Date: Wed, 20 Jul 2011 12:30:06 -0400
From: Tom McNemar <TMcNemar <@t> lmhealth.org>
Subject: RE: [Histonet] Controls with patient specimen on same slide
To: "'Rathborne, Toni'" <trathborne <@t> somerset-healthcare.com>, 'Richard
Cartun' <Rcartun <@t> harthosp.org>, "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
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<E9A90E28259D2F4E84308C5E8EA8F7B4381967AD21 <@t> lmhs-exchange.lmhealth.org>
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Each case gets one positive control per antibody. If, for example, I run a bone marrow bx and aspirate, I use one positive control for both slides.
Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcnemar <@t> lmhealth.org
www.LMHealth.org
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni
Sent: Wednesday, July 20, 2011 9:19 AM
To: 'Richard Cartun'; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Controls with patient specimen on same slide
All of these responses are great. So here's a follow up question.
Do you place a control tissue on EACH slide if you have multiple blocks for a case, or just on one of the slides?
-----Original Message-----
From: Richard Cartun [mailto:Rcartun <@t> harthosp.org]
Sent: Tuesday, July 19, 2011 6:40 PM
To: histonet <@t> lists.utsouthwestern.edu; Rathborne, Toni
Subject: Re: [Histonet] Controls with patient specimen on same slide
We do not put our positive control tissue on the test slide; we run batch controls. Many of the unstained slides (breast, GI, and prostate biopsies) that we use for IHC testing are cut in our Histology Laboratory as part of a part-type slide protocol. For example, we cut 7 slides, 2 sections on each slide, for breast biopsies and stain #1, 4, and 7 with H&E, and then use (if needed) #2, 3, 5, and 6 for IHC. Therefore, it would be very difficult for us to place the positive control tissue on the same slide. In addition, I receive a lot of consult cases from other hospitals where they send us unstained slides for testing. Once again, it would be difficult to place the positive control tissue on the same slide and it would slow us down in terms of starting those slides once they arrive. However, I think the main reason we don't pursue putting the positive control tissue on the same slide is the fact that it would consume an enormous amount of control tissue.
Richard
Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital
80 Seymour Street
Hartford, CT 06102
(860) 545-1596 Office
(860) 545-2204 Fax
>>> "Rathborne, Toni" <trathborne <@t> somerset-healthcare.com> 7/19/2011
>>> 3:27 PM >>>
Hi,
I'm interested in knowing how many of you are performing ihc with the control tissue and the patient tissue on the same slide. I have seen slides available which have designated areas for each tissue to be placed so there will not be any confusion. If you're doing it, have you encountered any problems? What benefits have you noticed since implementing this process? Are your pathologists in favor of this? If you're not, why not?
Thanks,
Toni
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This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you.
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Message: 8
Date: Wed, 20 Jul 2011 12:53:21 -0400
From: "Weems, Joyce" <JWeems <@t> sjha.org>
Subject: [Histonet] RE: Assisting with Autopsies
To: Amy Self <ASelf <@t> georgetownhospitalsystem.org>,
"'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<92AD9B20A6C38C4587A9FEBE3A30E164082122A73D <@t> CHEXCMS10.one.ads.che.org>
Content-Type: text/plain; charset="us-ascii"
We have two contract deiners.. They work at other hospitals around town and charge per case. Works well here as our numbers have decreased drastically over the years.
Joyce Weems
Pathology Manager
Saint Joseph's Hospital
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
678-843-7376 - Phone
678-843-7831 - Fax
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Amy Self
Sent: Tuesday, July 19, 2011 15:12
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] Assisting with Autopsies
Hello All,
We are small hospital that does approximately 5-10 autopsies a year. This being said our administration department does not want to hire a diener to assist with these autopsies. So I have decided to turn to all of you out there in histoland for a little poll.
Does your facility use histotechs or a diener to assist with the autopsy?
Thanks in advance for all of your help, Amy
Amy Self
Georgetown Hospital System
843-527-7179
NOTE:
The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer.
Thank you.
_______________________________________________
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Message: 9
Date: Wed, 20 Jul 2011 16:59:53 +0000
From: Nancy Schmitt <Nancy_Schmitt <@t> pa-ucl.com>
Subject: [Histonet] Controls with patient specimen on same slide
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<906B4DA90ED1DB4DB6C7E94D7CEE6C36791511 <@t> PEITHA.wad.pa-ucl.com>
Content-Type: text/plain; charset="us-ascii"
We place control tissue on EACH slide for IHC testing - even if multiple blocks from same case.
Nancy Schmitt HT, MLT(ASCP)
United Clinical Laboratories
-----------------------------------------
Message: 12
Date: Wed, 20 Jul 2011 13:18:52 +0000
From: "Rathborne, Toni" <trathborne <@t> somerset-healthcare.com>
Subject: RE: [Histonet] Controls with patient specimen on same slide
To: "'Richard Cartun'" <Rcartun <@t> harthosp.org>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<3AD061FE740D464FAC7BF6B5CFB75707031366 <@t> SMCMAIL01.somerset-healthcare.com>
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All of these responses are great. So here's a follow up question.
Do you place a control tissue on EACH slide if you have multiple blocks for a case, or just on one of the slides?
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Message: 4
Date: Wed, 20 Jul 2011 12:48:14 -0700 (PDT)
From: Adrienne Anderson <rennie1108 <@t> yahoo.com>
Subject: [Histonet] Research microtomes
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Cc: "bushma1 <@t> rose-hulman.edu" <bushma1 <@t> rose-hulman.edu>,
"mlosborn2 <@t> gmail.com" <mlosborn2 <@t> gmail.com>
Message-ID:
<1311191294.91032.YahooMailNeo <@t> web59614.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=us-ascii
Hello Histo-land,
I'm trying to find a microtome that can cut from 50-2000 micron sections. I've only had clinical experience, so I don't know of any such microtome. Any advice would be appreciated!
Thanks,
Adrienne
------------------------------
Message: 5
Date: Wed, 20 Jul 2011 16:02:19 -0500
From: Jack Ratliff <ratliffjack <@t> hotmail.com>
Subject: Re: [Histonet] Research microtomes
To: Adrienne Anderson <rennie1108 <@t> yahoo.com>
Cc: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>, "bushma1 <@t> rose-hulman.edu"
<bushma1 <@t> rose-hulman.edu>, "mlosborn2 <@t> gmail.com" <mlosborn2 <@t> gmail.com>
Message-ID: <BLU0-SMTP96DEBEFCD953A5410DD7CAE4C0 <@t> phx.gbl>
Content-Type: text/plain; charset="us-ascii"
What type of specimen are you trying to cut? What embedding media are you using?
Jack
On Jul 20, 2011, at 2:48 PM, Adrienne Anderson <rennie1108 <@t> yahoo.com> wrote:
> Hello Histo-land,
>
> I'm trying to find a microtome that can cut from 50-2000 micron sections. I've only had clinical experience, so I don't know of any such microtome. Any advice would be appreciated!
>
> Thanks,
> Adrienne
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
------------------------------
Message: 6
Date: Wed, 20 Jul 2011 14:57:48 -0700 (PDT)
From: Adrienne Anderson <rennie1108 <@t> yahoo.com>
Subject: Re: [Histonet] Research microtomes
To: Jack Ratliff <ratliffjack <@t> hotmail.com>
Cc: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>, "bushma1 <@t> rose-hulman.edu"
<bushma1 <@t> rose-hulman.edu>, "mlosborn2 <@t> gmail.com" <mlosborn2 <@t> gmail.com>
Message-ID:
<1311199068.60287.YahooMailNeo <@t> web59611.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=us-ascii
Hi Jack,
We're trying to cut just plain old FFPE blocks.
________________________________
From: Jack Ratliff <ratliffjack <@t> hotmail.com>
To: Adrienne Anderson <rennie1108 <@t> yahoo.com>
Cc: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>; "bushma1 <@t> rose-hulman.edu" <bushma1 <@t> rose-hulman.edu>; "mlosborn2 <@t> gmail.com" <mlosborn2 <@t> gmail.com>
Sent: Wednesday, July 20, 2011 5:02 PM
Subject: Re: [Histonet] Research microtomes
What type of specimen are you trying to cut? What embedding media are you using?
Jack
On Jul 20, 2011, at 2:48 PM, Adrienne Anderson <rennie1108 <@t> yahoo.com> wrote:
> Hello Histo-land,
>
> I'm trying to find a microtome that can cut from 50-2000 micron sections. I've only had clinical experience, so I don't know of any such microtome. Any advice would be appreciated!
>
> Thanks,
> Adrienne
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
------------------------------
Message: 7
Date: Wed, 20 Jul 2011 17:13:43 -0500
From: Jack Ratliff <ratliffjack <@t> hotmail.com>
Subject: Re: [Histonet] Research microtomes
To: Adrienne Anderson <rennie1108 <@t> yahoo.com>
Cc: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>, "bushma1 <@t> rose-hulman.edu"
<bushma1 <@t> rose-hulman.edu>, "mlosborn2 <@t> gmail.com" <mlosborn2 <@t> gmail.com>
Message-ID: <BLU0-SMTP1328EFABCD05A07BE45E5D1AE4C0 <@t> phx.gbl>
Content-Type: text/plain; charset="us-ascii"
Couple more questions. :) What is the tissue and the dimensions of the specimen?
On Jul 20, 2011, at 4:57 PM, Adrienne Anderson <rennie1108 <@t> yahoo.com> wrote:
> Hi Jack,
>
> We're trying to cut just plain old FFPE blocks.
>
> From: Jack Ratliff <ratliffjack <@t> hotmail.com>
> To: Adrienne Anderson <rennie1108 <@t> yahoo.com>
> Cc: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>; "bushma1 <@t> rose-hulman.edu" <bushma1 <@t> rose-hulman.edu>; "mlosborn2 <@t> gmail.com" <mlosborn2 <@t> gmail.com>
> Sent: Wednesday, July 20, 2011 5:02 PM
> Subject: Re: [Histonet] Research microtomes
>
> What type of specimen are you trying to cut? What embedding media are you using?
>
> Jack
>
>
>
> On Jul 20, 2011, at 2:48 PM, Adrienne Anderson <rennie1108 <@t> yahoo.com> wrote:
>
> > Hello Histo-land,
> >
> > I'm trying to find a microtome that can cut from 50-2000 micron sections. I've only had clinical experience, so I don't know of any such microtome. Any advice would be appreciated!
> >
> > Thanks,
> > Adrienne
> > _______________________________________________
> > Histonet mailing list
> > Histonet <@t> lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> >
>
>
------------------------------
Message: 8
Date: Wed, 20 Jul 2011 18:45:19 -0400
From: Bob Richmond <rsrichmond <@t> gmail.com>
Subject: [Histonet] embalmers using formaldehyde - NYT story
To: patho-l <@t> mailman.srv.ualberta.ca,
"Histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<CAOKsRH7OiVHwtgyTKJ0Q-vHs41LdDP04HY+zocchcku4xDvCXg <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
This New York Times story about the use of formaldehyde by embalmers
is worth reading by pathologists and histotechnologists.
http://www.nytimes.com/2011/07/21/business/despite-cancer-risk-embalmers-stay-with-formaldehyde.html?_r=1&hp
Seems like they've basically got the right idea.
Bob Richmond
Samurai Pathologist
Knoxville TN
------------------------------
Message: 9
Date: Wed, 20 Jul 2011 15:45:47 -0700 (PDT)
From: Joseph Saby <saby_joseph_a <@t> yahoo.com>
Subject: Re: [Histonet] Microtome draft shield
To: "Rathborne, Toni" <trathborne <@t> somerset-healthcare.com>, 'Keri
Colwell' <Keri.Colwell <@t> inspection.gc.ca>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<1311201947.45523.YahooMailNeo <@t> web114401.mail.gq1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
I would suggest that you look inthe local yellow pages for people who work with plexiglass.? This is a re;atively inexpensive medium.? You can design what you need and have your draft shields built to order.
?
Joe Saby
NAMSA
From: "Rathborne, Toni" <trathborne <@t> somerset-healthcare.com>
To: 'Keri Colwell' <Keri.Colwell <@t> inspection.gc.ca>; "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
Sent: Tuesday, July 19, 2011 9:11 AM
Subject: RE: [Histonet] Microtome draft shield
Haven't seen anything that specific, but you might want to look at the various biohazard splash guards. They are a clear Plexiglas, and they have a base to support them. You would be able to move them around (or have them mounted to the counter if desired), and they come in an assortment of angles and sizes.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Keri Colwell
Sent: Monday, July 18, 2011 5:21 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Microtome draft shield
Hi Everyone,
I work in a lab which is under negative pressure (air is continuously flowing in), and due to the layout of the rooms are microtomes are located next to two different doorways.? We are looking for some sort of draft shield to place around each microtome and water bath that will reduce the effects of the airflow and personnel movements on our ribbons.
Anyone have any suggestions as to who might sell such a thing?
Thanks in advance!
Keri Colwell
Laboratory Technologist | Technologiste de laboratoire TSE and Pathology Lethbridge Laboratory | Laboratoire de Lethbridge Canadian Food Inspection Agency | Agence candienne d'inspection des aliments Township Road 9-1 | Ch de Canton 9-1 Box 640? | CP 640 Lethbridge, AB T1J 3Z4 E-mail | Courriel: keri.colwell <@t> inspection.gc.ca Telephone | T?l?phone:? 403-382-5500 Facsimile | T?l?copieur: 403-382-5583 Government of Canada | Gouvernement du Canada
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------------------------------
Message: 10
Date: Thu, 21 Jul 2011 09:11:33 -0400
From: Denise Piontek <dbpiontek <@t> hotmail.com>
Subject: [Histonet] Re:
To: <camille_diges <@t> bio-rad.com>, <cdiges <@t> gmail.com>,
<canvaspeople <@t> email.canvaspeople.com>, <dpantano <@t> hrecruiters.com>,
<deedra711 <@t> yahoo.com>, <histonet <@t> lists.utsouthwestern.edu>,
<info <@t> littlepeopleplayhouse.com>
Message-ID: <COL112-W55A0B140D232A1B964ABF0AB4F0 <@t> phx.gbl>
Content-Type: text/plain; charset="iso-8859-1"
Sexual problems are to be solved... http://ravera.todouy.com/bestsite.php?opage=20f0
------------------------------
Message: 11
Date: Thu, 21 Jul 2011 07:49:22 -0700 (PDT)
From: Adrienne Anderson <rennie1108 <@t> yahoo.com>
Subject: Re: [Histonet] Research microtomes
To: Jack Ratliff <ratliffjack <@t> hotmail.com>
Cc: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>, "bushma1 <@t> rose-hulman.edu"
<bushma1 <@t> rose-hulman.edu>, "mlosborn2 <@t> gmail.com" <mlosborn2 <@t> gmail.com>
Message-ID:
<1311259762.22661.YahooMailNeo <@t> web59603.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=us-ascii
This post is actually on behalf of some interns that are working at my company over the summer. Their emails are included on this post, so I'm going to turn it over to them:) But here is their description of what they're doing:
"In answer to Jack's questions (I thought about replying myself, but didn't want to confuse anyone), we want to make a CEMA array (cutting edge matrix assembly array), which could be done with a variety of tissue types. The step that calls for the special microtome is when you cut the original blocks from the hospital -- meaning variable tissue depth.
Though the article mentioned a microtome that could cut 50-2000, we are most interested in the range around 50-150 microns."
Thanks,
Adrienne
________________________________
From: Jack Ratliff <ratliffjack <@t> hotmail.com>
To: Adrienne Anderson <rennie1108 <@t> yahoo.com>
Cc: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>; "bushma1 <@t> rose-hulman.edu" <bushma1 <@t> rose-hulman.edu>; "mlosborn2 <@t> gmail.com" <mlosborn2 <@t> gmail.com>
Sent: Wednesday, July 20, 2011 6:13 PM
Subject: Re: [Histonet] Research microtomes
Couple more questions. :) What is the tissue and the dimensions of the specimen?
On Jul 20, 2011, at 4:57 PM, Adrienne Anderson <rennie1108 <@t> yahoo.com> wrote:
Hi Jack,
>
>We're trying to cut just plain old FFPE blocks.
>
>
>
>________________________________
>From: Jack Ratliff <ratliffjack <@t> hotmail.com>
>To: Adrienne Anderson <rennie1108 <@t> yahoo.com>
>Cc: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>; "bushma1 <@t> rose-hulman.edu" <bushma1 <@t> rose-hulman.edu>; "mlosborn2 <@t> gmail.com" <mlosborn2 <@t> gmail.com>
>Sent: Wednesday, July 20, 2011 5:02 PM
>Subject: Re: [Histonet] Research microtomes
>
>What type of specimen are you trying to cut? What embedding media are you using?
>
>Jack
>
>
>
>On Jul 20, 2011, at 2:48 PM, Adrienne Anderson <rennie1108 <@t> yahoo.com> wrote:
>
>> Hello Histo-land,
>>
>> I'm trying to find a microtome that can cut from 50-2000 micron sections. I've only had clinical experience, so I don't know of any such microtome. Any advice would be appreciated!
>>
>> Thanks,
>> Adrienne
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>
>
>
------------------------------
Message: 12
Date: Thu, 21 Jul 2011 10:07:09 -0500
From: Jack Ratliff <ratliffjack <@t> hotmail.com>
Subject: Re: [Histonet] Research microtomes
To: Adrienne Anderson <rennie1108 <@t> yahoo.com>
Cc: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>, "bushma1 <@t> rose-hulman.edu"
<bushma1 <@t> rose-hulman.edu>, "mlosborn2 <@t> gmail.com" <mlosborn2 <@t> gmail.com>
Message-ID: <BLU0-SMTP12232DA09F9CB6EA8CFD67DAE4F0 <@t> phx.gbl>
Content-Type: text/plain; charset="us-ascii"
Thanks a lot for your response Adrienne! I think I may be able to help you with this. Is there a time we can talk off-line over the phone? My number is 317-281-1975.
Best Regards,
Jack
On Jul 21, 2011, at 9:49 AM, Adrienne Anderson <rennie1108 <@t> yahoo.com> wrote:
> This post is actually on behalf of some interns that are working at my company over the summer. Their emails are included on this post, so I'm going to turn it over to them:) But here is their description of what they're doing:
>
> "In answer to Jack's questions (I thought about replying myself, but didn't want to confuse anyone), we want to make a CEMA array (cutting edge matrix assembly array), which could be done with a variety of tissue types. The step that calls for the special microtome is when you cut the original blocks from the hospital -- meaning variable tissue depth.
>
> Though the article mentioned a microtome that could cut 50-2000, we are most interested in the range around 50-150 microns."
>
> Thanks,
> Adrienne
>
> From: Jack Ratliff <ratliffjack <@t> hotmail.com>
> To: Adrienne Anderson <rennie1108 <@t> yahoo.com>
> Cc: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>; "bushma1 <@t> rose-hulman.edu" <bushma1 <@t> rose-hulman.edu>; "mlosborn2 <@t> gmail.com" <mlosborn2 <@t> gmail.com>
> Sent: Wednesday, July 20, 2011 6:13 PM
> Subject: Re: [Histonet] Research microtomes
>
> Couple more questions. :) What is the tissue and the dimensions of the specimen?
>
>
>
> On Jul 20, 2011, at 4:57 PM, Adrienne Anderson <rennie1108 <@t> yahoo.com> wrote:
>
>> Hi Jack,
>>
>> We're trying to cut just plain old FFPE blocks.
>>
>> From: Jack Ratliff <ratliffjack <@t> hotmail.com>
>> To: Adrienne Anderson <rennie1108 <@t> yahoo.com>
>> Cc: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>; "bushma1 <@t> rose-hulman.edu" <bushma1 <@t> rose-hulman.edu>; "mlosborn2 <@t> gmail.com" <mlosborn2 <@t> gmail.com>
>> Sent: Wednesday, July 20, 2011 5:02 PM
>> Subject: Re: [Histonet] Research microtomes
>>
>> What type of specimen are you trying to cut? What embedding media are you using?
>>
>> Jack
>>
>>
>>
>> On Jul 20, 2011, at 2:48 PM, Adrienne Anderson <rennie1108 <@t> yahoo.com> wrote:
>>
>> > Hello Histo-land,
>> >
>> > I'm trying to find a microtome that can cut from 50-2000 micron sections. I've only had clinical experience, so I don't know of any such microtome. Any advice would be appreciated!
>> >
>> > Thanks,
>> > Adrienne
>> > _______________________________________________
>> > Histonet mailing list
>> > Histonet <@t> lists.utsouthwestern.edu
>> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>> >
>>
>>
>
>
------------------------------
Message: 13
Date: Thu, 21 Jul 2011 08:34:10 -0700 (PDT)
From: Sheila Haas <micropathlabs <@t> yahoo.com>
Subject: [Histonet] Small Slide Scanner
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<1311262450.88288.YahooMailRC <@t> web161715.mail.bf1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Hi all. Is anyone out there using one of the small, low volume slide scanners
for use in?frozen sections? We have?looked at a couple of the larger scanners
for other purposes but?wanted to know how the smaller ones would?work for our
pathologists to?review frozen slides from off-site facilities. Of course, a PA
would gross?the specimen at the facility,?cut the slides and?then load them into
the scanner. Any thoughts?
?
Sheila Haas
Laboratory Supervisor
MicroPath Laboratories, Inc.
------------------------------
Message: 14
Date: Thu, 21 Jul 2011 12:36:59 -0400
From: carpj <carpj <@t> wadsworth.org>
Subject: [Histonet] Fast Blue stabilization
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <A37574E6-6CB6-4F02-9955-A59C84837482 <@t> wadsworth.org>
Content-Type: text/plain; charset=iso-8859-1
Hi List,
We are retrogradely labeling spinal motoneurons with Fast Blue, which works well. When we process our sections (60-?m) for immunohistochemical labeling, the Fast Blue signal becomes much weaker. We believe that the detergents we are using to facilitate antibody penetration are also allowing the Fast Blue to leak out of the cells. Is anyone aware of methods for stabilizing Fast Blue within the cells so it won't wash out during immunohistochemical processing?
Jonathan Carp, Ph.D.
Wadsworth Center
New York State Dept. Health
Albany, NY
IMPORTANT NOTICE: This e-mail and any attachments may contain
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