[Histonet] Immuno. confusion

[Hannen, Valerie]

Hi folks...

I am going to try to revamp out Immuno. procedure and am looking at a couple of spec. sheets from our primary vendor. I am gettin conflicting information...so I am turning to you all for help.

When doing immuno's that require either HIER or enzyme digestion, do you perform the retrieval first or do you do the "peroxide" step first??
One spec. sheets says retrieve then block endogenous peroxidase...the other says block endogenous peroxidase then do the HIER or enzyme digestion..which is correct??

One other question that I have is...what do you use to enhance your stain?? The enhancing solution that we were using has been discontinued.

Thanks so much!!

Valerie Hannen,MLT(ASCP), HTL,SU(FL)
Histotechnologist
Parrish Medical Center
951 N. Washington Avenue
Titusville, Florida 32796
(321) 268-6111 ext. 7506



**************************************************************
"This email is intended solely for the use of the individual to
whom it is addressed and may contain information that is
privileged, confidential or otherwise exempt from disclosure
under applicable law. If the reader of this email is not the
intended recipient or the employee or agent responsible for
delivering the message to the intended recipient, you are
hereby notified that any dissemination, distribution, or
copying of this communication is strictly prohibited. If you
have received this communication in error, please immediately
delete this message. Thank you"
**************************************************************