[Histonet] Processing of derm specimens

CrochiereSteve <@t> aol.com CrochiereSteve <@t> aol.com
Tue Aug 2 17:46:20 CDT 2011


I have recently had a problem with my skin specimens being  
"underprocessed". I use a Leica 300ASP. The schedule is as follows:
10% NBF x 2 for 1 hour ea.
80% Reagent Alcohol for 1 hour
95% Reagent Alcohol x 2 for 1 hour each
100% Reagent Alcohol for 1 hour each
Xylene x 3 for 1 hour each
Paraffin x 3 for 1 hour each
 
The specimens are "mushy and swell on the ice"
Any input is welcome.
 
send response to :
_scrochiere <@t> nedlc.com_ (mailto:scrochiere <@t> nedlc.com) 
 
thanks


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