[Histonet] Re: C4d stain for IF

[Patrick Laurie]

Try the C4d directly labeled antibody from alpco
www.alpco.com

anti human C4d  (FITC-Conjugated)
Cat #:   04-BI-RC4D-FITC

Good luck.

On Wed, Apr 27, 2011 at 2:54 PM, Butler, Roszetta <rbutler <@t> ameripath.com> wrote:
> I'm having trouble with my C4d IF stain.  I'm using AbDirect/Serotec, mouse monoclonal diluted 1:500 for 1 hr, and the Vector is AbDirect/Serotec  diluted 1:25 for ½ hour.  This is not working and I've tried different dilutions with no success.  Can anyone share their protocol?
>
> Rosie Butler,
> AmeriPath/3000 United Founders Blvd./Suite 234/Oklahoma City, OK 73112/405.842.7575 - Office
> 405.650.8921 - Cell/405.841.2002 - Fax
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-- 
Patrick Laurie HT(ASCP)QIHC
CellNetix Pathology & Laboratories
1124 Columbia Street, Suite 200
Seattle, WA 98104
plaurie <@t> cellnetix.com