[Histonet] How to keep paraffin sections from sticking toblade/blade holder.

[Kuhnla, Melissa]

Check the retraction on the back of the mictrotome. Switch to the other setting

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Thursday, April 21, 2011 3:26 PM
To: Histonet list serv.; BretClough
Subject: Re: [Histonet] How to keep paraffin sections from sticking toblade/blade holder.

1- change cutting angle
2- keep the blade holder clean (without paraffin)
3- make sure the blade is not blunt
René J.
 
 

--- On Thu, 4/21/11, Clough, Bret <Clough <@t> medicine.tamhsc.edu> wrote:


From: Clough, Bret <Clough <@t> medicine.tamhsc.edu>
Subject: [Histonet] How to keep paraffin sections from sticking to blade/blade holder.
To: "Histonet list serv." <histonet <@t> lists.utsouthwestern.edu>
Date: Thursday, April 21, 2011, 3:07 PM


While sectioning paraffin embedded tissue on a rotary  microtome I noticed the sections sticking to the blade holder and bunching up. What do I need to do so that my section come off the blade in a ribbon? Please note that this is my first time in sectioning tissue and this is a learning exercise  for me.

Thanks in advance for your suggestions .

Bret
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