[Histonet] How to keep paraffin sections from sticking to blade/blade holder.

Clough, Bret Clough <@t> medicine.tamhsc.edu
Thu Apr 21 14:07:54 CDT 2011


While sectioning paraffin embedded tissue on a rotary  microtome I noticed the sections sticking to the blade holder and bunching up. What do I need to do so that my section come off the blade in a ribbon? Please note that this is my first time in sectioning tissue and this is a learning exercise  for me.

Thanks in advance for your suggestions .

Bret


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