[Histonet] IHC validation

[Troutman, Kenneth A]

Hematoxylin should not affect the immunoreactivity (since counterstaining occurs following the IHC reaction), so no, changing the hematoxylin would not require a revalidation.  Just make sure you are not obscuring the immunostain with an overly-dark counterstain.  The point is to provide contrast between the DAB (brown) or fast red chromagen and to offer the reader a sense of context of the surrounding morphology of the tissue being stained.

Ashley Troutman BS, HT(ASCP) QIHC
Immunohistochemistry Supervisor
Vanderbilt University Histopathology
1301 Medical Center Drive TVC 4531
Nashville, TN  37232

Message: 10
Date: Tue, 19 Apr 2011 14:04:32 -0500
From: "Ring, Mary L" <Mary.L.Ring <@t> HealthPartners.Com>
Subject: [Histonet] IHC validation
To: "histonet <@t> lists.utsouthwestern.edu"
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Does a change in the type of hematoxylin counterstain require any re-validation of IHC stains?
Thanks for your help!

Mary Ring, HT, QIHC
Regions Hospital, St Paul, Mn