[Histonet] trichrome/ frozen cardiac tissue

Molinari, Betsy BMolinari <@t> heart.thi.tmc.edu
Thu Apr 21 07:25:24 CDT 2011


Hi,
 I have some frozen house hearts that were perfused with 4% paraformaldehyde. I found protocols for FF, but not fixed frozen. I tried staining them with my usual Gomori I use on my paraffin blocks but the doc thinks they picked up too much red, not enough green.
Any suggerstions..I need to get them done today, they were brought up to RT this morning.
Thanks.

Betsy Molinari HT(ASCP)
Texas Heart Institute
Cardiovascular Pathology
6770 Bertner Ave
MC1-283
Houston,TX 77030-2607
832-355-6524
832-355-6812



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