[Histonet] [Histonet Biopsy Program for Sakura VIP 5/6 Processor

[Rene J Buesa]

Incidentally, using ethanol + isopropanol makes no sense and has no chemical fundament.
Results are better using isopropanol ONLY.
René J.

--- On Tue, 4/19/11, Mayer,Toysha N <TNMayer <@t> mdanderson.org> wrote:


From: Mayer,Toysha N <TNMayer <@t> mdanderson.org>
Subject: [Histonet] [Histonet Biopsy Program for Sakura VIP 5/6 Processor
To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
Date: Tuesday, April 19, 2011, 11:47 AM


Try to eliminate one of the 100% Alcohols.  If it cannot eliminate it, then just pump it in and out.
Microwave processing actually uses Ethanol, Isopropyl, and paraffin only.


Toysha N. Mayer, MBA, HT (ASCP)
Education Coordinator
Program in Histotechnology
School of Health Professions
MD Anderson Cancer Center
(713) 563-3481
tnmayer <@t> mdanderson.org


> Message: 8
> Date: Tue, 12 Apr 2011 14:57:18 -0400
> From: "Evans, Andria B" <aevans3 <@t> lghealth.org>
> Subject: [Histonet] Biopsy program for Sakura VIP 5/6 Processor
> To: "histonet <@t> lists.utsouthwestern.edu"
>     <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
>      
> <4182FDF23D7C9948BC41C4C082C3A54F021557B3AA60 <@t> MAIL-AG-CLUSTER.lha.org>
> Content-Type: text/plain;    charset="iso-8859-1"
>
> Our lab is currently looking for a way to shorten our Biopsy 
> processing program without compromising the patient specimen.  We do 
> have an issue with our GI's being very dry, which causes us to have to 
> soak between each level taken and also causes a lot of chatter.  Also 
> we have a goal to do a run during the day to improve turn around time. 
> Here is what our current protocol is....
>
> Formalin    1 min       37degrees
> Formalin    15 mins    37degrees
> 70             14 mins    40 degrees
> 95             14 mins    40 degrees
> 95             9 mins      40 degrees
> 100           9 mins      40 degrees
> 100           7 mins      40 degrees
> 100           4 mins      40 degrees
> Xylene       23 mins    no heat
> Xylene       15 mins    no heat
> Paraffin      20 mins   60 degrees
> Paraffin      18 mins   60 degrees
> Paraffin      10 mins   60 degrees
> Paraffin      0 mins     60 degrees
>
> All the steps are set on a fast mix setting.  All of our biopsy 
> specimens are put into sponges.
>
> Any feedback would be greatly appreciated.
>
> Andria B Evans HTL(ASCP)CM
> Lancaster General Hospital
> 555 North Duke Street
> Lancaster, PA  17604
> (717)544-5511 ext: 77329
> aevans3 <@t> lgheath.org<mailto:aevans3 <@t> lgheath.org>

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