[Histonet] Cell Block IHC

[sgoebel <@t> mirnarx.com]

Hey ya'll,

So I am trying to do IHC on paraffin embedded cell blocks.  The cell
blocks were made in an agar solution.  The H&E slides came out fine and
look like a normal cell block slide.  However I am now a little
confused.  I put the slides through HIER and it doesn't look like there
is anything on the slide anymore?  Are the cells still there, just the
agar fell off because of the heat?  Should I continue to use all the
reagents and antibody on these slides?  Also, if they are trash...how do
I do IHC stains on these blocks?  I do not have access to a cytospin
machine so my "traditional" way of doing ICC with cytospins I cannot do.
Ahhh!!  Help!!!

 

Sarah Goebel, BA, HT(ASCP)

Histotechnologist

Mirna Therapeutics

2150 Woodward Street

Suite 100

Austin, Texas  78744

(512)901-0900 ext. 6912