[Histonet] Tissue transfer

[Kuhnla, Melissa]

Hello,
We do have a successful way to transfer tissue sections.  It is using Mount Quik liquid coverslip. We purchase this from Newcomber Supply. Are you doing IHC testing?
1. H&E sections first (so they are visible)(for IHC, retrieval will fade the stain anyways)
2. After the stain, run slide to Xylene
3. Cover entire slide with layer of Mount Quik
4. Bake horizontal in 60 degree oven for 1 hour
5. Bake vertical for one hour
6. Rinse slide in running warm water until the mount quik and tissue lift from the slide
7. Divide the tissue as need, place portions on plus slide
8. Bake horizontal for 1 hour
9. Bake vertical for one hour
10. Dissolve off mount quik with several 10 minute steps in Xylene. No visible residual should be left
11. Carry out testing

Hope this helps

Melissa

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Wednesday, April 13, 2011 3:54 PM
To: histonet <@t> lists.utsouthwestern.edu; Jeanine (CDC/OID/NCEZID)Bartlett
Subject: Re: [Histonet] Tissue transfer

"Mission impossible" (successfully, you can always ruin them)! 
It is better to do the staining after a prolonged oven heating and do it delicately.
René J.

--- On Wed, 4/13/11, Bartlett, Jeanine (CDC/OID/NCEZID) <jqb7 <@t> cdc.gov> wrote:


From: Bartlett, Jeanine (CDC/OID/NCEZID) <jqb7 <@t> cdc.gov>
Subject: [Histonet] Tissue transfer
To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
Date: Wednesday, April 13, 2011, 1:06 PM


Hello all,

We have some unstained slides we received from outside the U.S. on non-charged/un-coated slides. We would like to transfer these sections to charged slides before proceeding with testing.  Any suggestions?

Thanks!

Jeanine Bartlett, BS, HT(ASCP)QIHC
Centers for Disease Control and Prevention
Infectious Diseases Pathology Branch
1600 Clifton Road, MS/G-32
18/SB-114
Atlanta, GA  30333
(404) 639-3590
jeanine.bartlett <@t> cdc.hhs.gov



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