[Histonet] RE: Biopsy program for Sakura VIP 5/6 Processor

[WILLIAM DESALVO]

I would consider replacing the sponges. Tony is correct that you need to make sure the air is removed from the sponge to facilitate exchange, but with shortened processing times you will undoubtedly cause carry over from solution to solution. Yuo may want to have a small container of alcoholic formalin with sponges sitting on the gross table.

Try using a nylon tissue bag to funnel filter your small biopsies. all your biopsy sample sizes can be placed into the bag and very tiny samples are retained. Last suggestion is to consider a folded filter paper method (we name it origami, I can provide directly if you want) that uses a quick four fold process w/ forceps at the gross bench to create a pocket for the tiny samples, has a single layer between the tissue/solution and is easy to open at embedding (without "popping" of tissue samples). Avoind the unnecessary carryover of the sponge if you can.

William DeSalvo, B.S., HTL(ASCP)





> From: AnthonyH <@t> chw.edu.au
> To: aevans3 <@t> lghealth.org; histonet <@t> lists.utsouthwestern.edu
> Date: Tue, 12 Apr 2011 23:30:30 +0000
> CC: 
> Subject: [Histonet] RE: Biopsy program for Sakura VIP 5/6 Processor
> 
> Increase the fixation time and make sure the air is out of the sponges (will stop formalin from getting to the tissue- a quick dunk of the cassette (with sponge and tissue) in alcohol and back into formalin will do the trick).
> 
> Regards 
> Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
> Laboratory Manager & Senior Scientist 
> Tel: 612 9845 3306 
> Fax: 612 9845 3318 
> the children's hospital at westmead
> Cnr Hawkesbury Road and Hainsworth Street, Westmead
> Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 
> 
> 
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Evans, Andria B
> Sent: Wednesday, 13 April 2011 4:57 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Biopsy program for Sakura VIP 5/6 Processor
> 
> Our lab is currently looking for a way to shorten our Biopsy processing program without compromising the patient specimen.  We do have an issue with our GI's being very dry, which causes us to have to soak between each level taken and also causes a lot of chatter.  Also we have a goal to do a run during the day to improve turn around time.   Here is what our current protocol is....
> 
> Formalin    1 min       37degrees
> Formalin    15 mins    37degrees
> 70             14 mins    40 degrees
> 95             14 mins    40 degrees
> 95             9 mins      40 degrees
> 100           9 mins      40 degrees
> 100           7 mins      40 degrees
> 100           4 mins      40 degrees
> Xylene       23 mins    no heat
> Xylene       15 mins    no heat
> Paraffin      20 mins   60 degrees
> Paraffin      18 mins   60 degrees
> Paraffin      10 mins   60 degrees
> Paraffin      0 mins     60 degrees
> 
> All the steps are set on a fast mix setting.  All of our biopsy specimens are put into sponges.
> 
> Any feedback would be greatly appreciated.
> 
> Andria B Evans HTL(ASCP)CM
> Lancaster General Hospital
> 555 North Duke Street
> Lancaster, PA  17604
> (717)544-5511 ext: 77329
> aevans3 <@t> lgheath.org<mailto:aevans3 <@t> lgheath.org>
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