[Histonet] RE: Biopsy program for Sakura VIP 5/6 Processor
Tony Henwood
AnthonyH <@t> chw.edu.au
Tue Apr 12 18:30:30 CDT 2011
Increase the fixation time and make sure the air is out of the sponges (will stop formalin from getting to the tissue- a quick dunk of the cassette (with sponge and tissue) in alcohol and back into formalin will do the trick).
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Evans, Andria B
Sent: Wednesday, 13 April 2011 4:57 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Biopsy program for Sakura VIP 5/6 Processor
Our lab is currently looking for a way to shorten our Biopsy processing program without compromising the patient specimen. We do have an issue with our GI's being very dry, which causes us to have to soak between each level taken and also causes a lot of chatter. Also we have a goal to do a run during the day to improve turn around time. Here is what our current protocol is....
Formalin 1 min 37degrees
Formalin 15 mins 37degrees
70 14 mins 40 degrees
95 14 mins 40 degrees
95 9 mins 40 degrees
100 9 mins 40 degrees
100 7 mins 40 degrees
100 4 mins 40 degrees
Xylene 23 mins no heat
Xylene 15 mins no heat
Paraffin 20 mins 60 degrees
Paraffin 18 mins 60 degrees
Paraffin 10 mins 60 degrees
Paraffin 0 mins 60 degrees
All the steps are set on a fast mix setting. All of our biopsy specimens are put into sponges.
Any feedback would be greatly appreciated.
Andria B Evans HTL(ASCP)CM
Lancaster General Hospital
555 North Duke Street
Lancaster, PA 17604
(717)544-5511 ext: 77329
aevans3 <@t> lgheath.org<mailto:aevans3 <@t> lgheath.org>
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