[Histonet] RE: Biopsy program for Sakura VIP 5/6 Processor

[Tony Henwood]

Increase the fixation time and make sure the air is out of the sponges (will stop formalin from getting to the tissue- a quick dunk of the cassette (with sponge and tissue) in alcohol and back into formalin will do the trick).

Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Laboratory Manager & Senior Scientist 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Evans, Andria B
Sent: Wednesday, 13 April 2011 4:57 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Biopsy program for Sakura VIP 5/6 Processor

Our lab is currently looking for a way to shorten our Biopsy processing program without compromising the patient specimen.  We do have an issue with our GI's being very dry, which causes us to have to soak between each level taken and also causes a lot of chatter.  Also we have a goal to do a run during the day to improve turn around time.   Here is what our current protocol is....

Formalin    1 min       37degrees
Formalin    15 mins    37degrees
70             14 mins    40 degrees
95             14 mins    40 degrees
95             9 mins      40 degrees
100           9 mins      40 degrees
100           7 mins      40 degrees
100           4 mins      40 degrees
Xylene       23 mins    no heat
Xylene       15 mins    no heat
Paraffin      20 mins   60 degrees
Paraffin      18 mins   60 degrees
Paraffin      10 mins   60 degrees
Paraffin      0 mins     60 degrees

All the steps are set on a fast mix setting.  All of our biopsy specimens are put into sponges.

Any feedback would be greatly appreciated.

Andria B Evans HTL(ASCP)CM
Lancaster General Hospital
555 North Duke Street
Lancaster, PA  17604
(717)544-5511 ext: 77329
aevans3 <@t> lgheath.org<mailto:aevans3 <@t> lgheath.org>
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