[Histonet] Von kossa stain

[Jack Ratliff]

I purchase a kit from Dorn and Hart Microedge and use sodium carbonate-formaldehyde solution to chemically develop.

Stain in silver nitrate for 5 min in the dark, 3 fresh DI rinses for 1 min each in the dark, then sodium carbonate-formaldehyde solution for 2 min in the dark, 2 fresh DI rinses (normal light) for 1 min each, 30 seconds in Farmer's Diminisher (Sodium thiosulfate-potassium ferricyanide soluiton to stop reaction) and a running tap water rinse for 10 min.
 
Jack
 

 
> From: Dorothy.L.Webb <@t> HealthPartners.Com
> To: histonet <@t> lists.utsouthwestern.edu
> Date: Tue, 12 Apr 2011 12:35:18 -0500
> Subject: [Histonet] Von kossa stain
> 
> What is everyone using for their "light" when developing the silver in the VonKossa stain when you have no sunlight to use? We used to use a 60 watt lamp, but haven't done one for years and am bringing this stain back to our repetiore due to pathologist request. Thanks much!
> 
> Dorothy Webb, HT (ASCP)
> Regions Histology Technical Supervisor
> 651-254-2962
> 
> 
> 
> 
> ________________________________
> This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited.
> 
> If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. HealthPartners R001.0
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet