[Histonet] Re: Giemsa Stain for frozens

[Sherwood, Margaret]

Actually, Bob, the method the investigator sent me was the Romanovsky Giemsa
that uses HEPES buffer.  But it requires preparing all the reagents from scratch
and the HEPES alone is several steps. I would like to buy prepared reagents.
Can you buy a Giemsa kit for frozens?

Peggy 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Robert Richmond
Sent: Monday, April 04, 2011 2:07 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Re: Giemsa Stain for frozens

Peggy Sherwood with the Wellman Center for Photomedicine at the
Massachusetts General Hospital asks:

>>I'm curious what Giemsa stain method people are using for frozen sections? I
have a simple Wright-Giemsa stain that I use for blood smears (using a 1 min.
methanol fix, 30 second stain with Wright-Giemsa, 5 minute in Phosphate buffer
and then rinse in dH20). The investigator sent me a method using a HEPES buffer.
It is involved to make up the working buffer of HEPES.<<

If it LOOKS good, it IS good, as Duke Ellington might have said. In
surgical pathology most people use one of the two-part Romanovsky type
stains - Diff-Quik or any of a number of satisfactory generic
equivalents. This is a very simple stain to do. I would think it would
be publishable, since the stain is well known and easily obtained.

Bob Richmond
Samurai Pathologist
Knoxville TN

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