[Histonet] Re: Giemsa Stain for frozens

[Robert Richmond]

Peggy Sherwood with the Wellman Center for Photomedicine at the
Massachusetts General Hospital asks:

>>I'm curious what Giemsa stain method people are using for frozen sections? I have a simple Wright-Giemsa stain that I use for blood smears (using a 1 min. methanol fix, 30 second stain with Wright-Giemsa, 5 minute in Phosphate buffer and then rinse in dH20). The investigator sent me a method using a HEPES buffer. It is involved to make up the working buffer of HEPES.<<

If it LOOKS good, it IS good, as Duke Ellington might have said. In
surgical pathology most people use one of the two-part Romanovsky type
stains - Diff-Quik or any of a number of satisfactory generic
equivalents. This is a very simple stain to do. I would think it would
be publishable, since the stain is well known and easily obtained.

Bob Richmond
Samurai Pathologist
Knoxville TN