[Histonet] Giemsa Stain for frozens

[Sherwood, Margaret]

I'm curious what Giemsa stain method people are using for frozen sections?  I
have a simple Wright-Giemsa stain that I use for blood smears (using a 1 min.
methanol fix, 30 second stain with Wright-Giemsa, 5 minute in Phosphate buffer
and then rinse in dH20).  The investigator sent me a method using a HEPES
buffer.  It is involved to make up the working buffer of HEPES.  I would
appreciate any input.

Thanks!
Peggy

Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (EDR 214)
Massachusetts General Hospital
55 Fruit Street
Boston, MA 02114-2696
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-1206 (fax)
msherwood <@t> partners.org 




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