[Histonet] Kappa/Lambda ISH on Ventana

[Sebree Linda A]

I was informed of the ASR issue also.  Back in the day, vendors,
including VMS, did give out protocols for ASR probes but no longer.  The
best I can offer is that with the VMS EBER probe we use ISH Protease
2/12" with great results.
 

Linda A. Sebree 
University of Wisconsin Hospital & Clinics 
IHC/ISH Laboratory 
DB1-223 VAH 
600 Highland Ave. 
Madison, WI 53792 
(608)265-6596 

 

________________________________

From: Angela Bitting [mailto:akbitting <@t> geisinger.edu] 
Sent: Friday, April 01, 2011 8:42 AM
To: Steven Wilkes; histonet <@t> lists.utsouthwestern.edu; Sebree Linda A
Subject: RE: [Histonet] Kappa/Lambda ISH on Ventana


Ventana can no longer give out protocols. Theses probes are ASR products
and the FDA doesn't allow them to tell you the protocol. They can offer
guidance if you have a protocol that doesn't work well, but they can't
outright tell you a protocol. Another institution that runs Ventana
probes is your best bet. 
 
 
Angela Bitting, HT(ASCP), QIHC
Technical Specialist, Histology
Geisinger Medical Center 
100 N Academy Ave. MC 23-00
Danville, PA 17822
phone  570-214-9634
fax  570-271-5916 
 
No trees were hurt in the sending of this email
However many electrons were severly inconvienienced!

>>> "Sebree Linda A" <LSebree <@t> uwhealth.org> 3/31/2011 7:09 PM >>>
You should be able to get protocols directly from Ventana.  I tried out
their K and L ISH probes and they gave us the protocols.  They also
supplied us with the protocol we use for EBER ISH. 


Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Steven
Wilkes
Sent: Thursday, March 31, 2011 3:10 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Kappa/Lambda ISH on Ventana

Hi Histonet!

I have been tasked with validating Kappa and Lambda on our Ventana
Benchmark
XT machines.  Does anyone who uses this platform have suggestions as to
which Ventana protease works best (1, 2 or 3)?  Additionally, any other
information concerning the optimization (suggested incubation times, RNA
regions etc.) would be greatly appreciated.  Thanks in advance.

Steven
srwilkes <@t> gmail.com
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